[en] Blends of anhydrous milkfat (AMF) and linseed oil (70/30), and AMF, rapeseed oil (RO) and linseed oil (LO), 70/20/10, were submitted to enzymatic interesterification. The oxidative stability of the blends, the interesterified (IE) blends and IE blends with 50 ppm -tocopherol added as antioxidant were studied. Samples were stored in open flasks at 60°C, 25°C and 4°C, and periodically submitted to peroxide, p-anisidine, TBA value determination and UV measurement at 232 and 268 nm. The analysis of volatile compounds was carried out by SPME for the samples stored at 60°C. Peroxides appeared to be the only significant oxidation products after 12 weeks storage at 4°C. As expected, the binary blends (BB) were more sensitive to oxidation than the ternary blends (TB). The BB were associated with increased volatile emission compared to TB. Interesterification led to variable effects on the oxidation of fat mixtures, depending on composition and temperature (beneficial effect on BB, at both 25°C and 60°C, and a rather neutral effect on TB). The IE blends exhibited higher volatile release prior to ageing. A pro-oxidant effect of -tocopherol addition was observed at 25°C on both BB and TB. At 60°C, an antioxidant effect was observed on TB.
Adamczak, M. The applications of lipases in modifying the composition, structure and properties of lipids - a review. Pol. J. Food Nutr. Sci. 2004, 13, 3-10.
Augustin, M. A., Versteeg, C. Milk fat: physical, chemical and enzymatic modifications. In Advanced Dairy Chemistry, 3rd ed.; Fox. P. F., McSweeney, P. L. H., Eds.; Springer: New York, 2006; Vol. 2: Lipids, pp 293-331.
Hunter, J. E. Dietary levels of trans-fatty acids: basis for health concerns and industry efforts to limit use. Nutr. Res. (N. Y.) 2005, 25, 499-513.
Balcão, V. M.; Kemppinen, A.; Malcata, F. X.; Kalo, P. J. Modification of butterfat by selective hydrolysis and interesterification by lipase: process and product characterization. J. AOCS 1998, 75, 1347-1358.
Zhang, H.; Xu, X.; Mu, H.; Nilsson, J.; Adler-Nissen, J.; Høy, C.-E. Lipozyme IM-catalysed interesterification for the production of margarine fats in 1 kg scale stirred tank reactor. Eur. J. Lipid Sci. Tephnol. 2000, 102, 411-418.
Hamam, F.; Shahidi, F. Synthesis of structured lipids containing medium-chain and omega-3 fatty acids. J. Agric. Food Chem. 2006, 54, 4390-4396.
Osborn, H. T.; Akoh, C. C. Structured lipids - novel fats with medical, nutraceutical, and food applications. Compr. Rev. Food Sci. Food Saf. 2002, 3, 110-120.
Knothe, G. Autoxidation and photo-oxidation. In The Lipid Handbook, 3rd ed.; Gunstone. F. D., Harwood. J. L., Dijkstra, A. J.. Eds.; CRC Press: Boca Raton, FL, 2007; pp 535-542.
Turan, S.; Karabulut, I.; Vural, H. Influence of sn-1,3-lipasecatalysed interesterification on the oxidative stability of soybean oil-based structured lipids. J. Sci. Food Agric. 2007, 87, 90-97.
Kimoto, H.; Endo, Y.; Fujimoto, K. Influence of interesterification on the oxidative stability of marine oil triacylglvcerols. J. AOCS 1994, 71, 469-412.
Wijesundera, C.; Ceccato, C.; Watkins, P.; Fagan, P.; Fraser, B.; Thienthong. N.; Perlmutter, P. Docohexaenoic acid is more stable to oxidation when located at the sn-2 position of triacylglycerol compared to sn-1(3). J. AOCS 2008, 85, 543-548.
Aguedo, M.; Hanon, E.; Danthine, S.; Paquot, M.; Lognay, G.; Thomas, A.; Vandenbol, M.; Thonart. P.; Wathelet, J.-P. Blecker, C. Enrichment of anhydrous milk fat in polyunsaturated fatty acid residues form linseed and rapeseed oils through enzymatic interesterification. J. Agric. Food Chem. 2008, 56, 1757-1765.
Russin, T. A.; Boye, J. I.; Pham, H. M.; Arcand, Y. Antioxydant properties of genistein in a model edible oil system. Food Chem. Toxicol. 2006, 71, 395-399.
Official Methods and Recommended Practices of the American Oil Chemist's Society, 5th ed.; Firestone, D., Ed.; American Oil Chemists' Society: Champaign, IL, 1998.
Baba, N.; Horita, N.; Nakajima, S. Lipase activity for lipid hydroperoxides. Sci. Rep. Fac. Agric., Okayama Univ. 1998, 87, 65-69.
Gardner, H. W. Oxygen radical chemistry of polyunsaturated fatty acids. Free Radical Biol. Med. 1989, 7, 65-86.
Lund, P.; Holmer, G. Characterization of volatiles from cultured dairy spreads during storage by dynamic headspace GC/MS. Eur. Food Res. Technol. 2001, 212, 636-642.
Krist, S.; Stuebiger, G.; Bail, S.; Unterweg, H. Analysis of volatile compounds and triacylglycerol composition of fatty seed oil gained from flaw and false flax. Eur. J. Lipid Sci. Technol. 2006, 108, 48-60.
Widder, S.; Sen, A.; Grosch, W. Changes in the flavour of butter oil during storage. Identification of potent odorants. Z. Lebensm. Unters. Forsch. 1991, 193, 32-35.
Aguedo, M.; Giet. J.-M.; Hanon, E.; Lognay, G.; Wathelet, B.; Destain, J.; Brasseur, R.; Vandenbol, M.; Danthine, S.; Blecker, C.; Wathelet, J.-P. Calorimetric study of milk fat, rapeseed oil blends and of their interesterification products. Eur. J. Lipid Sci. Technol. 2009, 111, 376-385.
Kamal-Eldin, A.; Appelqvist, L.-Å. The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids 1996, 31, 671-701.
Frega, N.; Mozzon, M.; Lercker, G. Effects of free fatty acids on oxidative stability of vegetable oils. J. AOCS 1999, 76, 325-329.
Marinova, E. M.; Yanishlieva, N. V. Effect of temperature on the antioxidative action of inhibitors in lipid autoxidation. J. Sci. Food Agric. 1992, 60, 313-318.
Kamal-Eldin, A.; Mäkinen, M.; Lampi, A.-M.; Hopia, A. A multivariate study of α-tocopherol and hydroperoxyde interaction during the oxidation of methvl linoleate. Eur. Food Res. Technol. 2002, 214, 52-57.
Lampi, A.-M.; Kataja, L.; Kamal-Eldin, A.; Vieno, P. Antioxidant activities of α- and γ-tocopherols in the oxidation of rapeseed oil triacylglycerols. J. AOCS 1999, 76, 749-755.