Centa as a Chromogenic Substrate for Studying Beta-Lactamases

[en] CENTA, a chromogenic cephalosporin, is readily hydrolyzed by beta-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of beta-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Bebrone, Carine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Moali, C.

Mahy, F.

Rival, S.; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Docquier, Jean-Denis

Rossolini, G. M.

Fastrez, J.

Pratt, R. F.

Frère, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie

Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Language :

English

Title :

Centa as a Chromogenic Substrate for Studying Beta-Lactamases

Publication date :

June 2001

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

1868-71

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 March 2009

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Bibliography

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