Article (Scientific journals)
Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells.
Kerselidou, Despoina; Dohai, Bushra Saeed; Nelson, David R. et al.
2021In Science Advances, 7 (19)
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Abstract :
[en] The endoplasmic reticulum (ER) is a central eukaryotic organelle with a tubular network made of hairpin proteins linked by hydrolysis of guanosine triphosphate nucleotides. Among posttranslational modifications initiated at the ER level, glycosylation is the most common reaction. However, our understanding of the impact of glycosylation on the ER structure remains unclear. Here, we show that exostosin-1 (EXT1) glycosyltransferase, an enzyme involved in N-glycosylation, is a key regulator of ER morphology and dynamics. We have integrated multiomics and superresolution imaging to characterize the broad effect of EXT1 inactivation, including the ER shape-dynamics-function relationships in mammalian cells. We have observed that inactivating EXT1 induces cell enlargement and enhances metabolic switches such as protein secretion. In particular, suppressing EXT1 in mouse thymocytes causes developmental dysfunctions associated with the ER network extension. Last, our data illuminate the physical and functional aspects of the ER proteome-glycome-lipidome structure axis, with implications in biotechnology and medicine.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kerselidou, Despoina 
Dohai, Bushra Saeed
Nelson, David R.
Daakour, Sarah
De Cock, Nicolas 
Hassoun, Zahrat El Oula ;  Université de Liège - ULiège > GIGA Molecul. Biolog. of Diseases - Gene Expression & Cancer
Kim, Dae-Kyum
Olivet, Julien ;  Université de Liège - ULiège > GIGA
El Assal, Diana C.
Jaiswal, Ashish
Alzahmi, Amnah
Pain, Charlotte
Matthijssens, Filip
Lemaitre, Pierre
Herfs, Michael ;  Université de Liège - ULiège > GIGA Cancer - Experimental Pathology
Chapuis, Julien
Ghesquiere, Bart
Vertommen, Didier
Kriechbaumer, Verena
Knoops, Kèvin
Lopez-Iglesias, Carmen
van Zandvoort, Marc
Lambert, Jean-Charles
Hanson, Julien  ;  Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique
Desmet, Christophe  ;  Université de Liège - ULiège > GIGA I3 - Cellular and Molecular Immunology
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Lauersen, Kyle J.
Vidal, Marc
Van Vlierberghe, Pieter
Dequiedt, Franck  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et biologie moléculaires animales
Salehi-Ashtiani, Kourosh
Twizere, Jean-Claude  ;  Université de Liège - ULiège > GIGA Molecul. Bio. of Diseases - Viral Interactomes Network
More authors (23 more) Less
Language :
English
Title :
Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells.
Publication date :
2021
Journal title :
Science Advances
eISSN :
2375-2548
Publisher :
American Association for the Advancement of Science (AAAS), Washington, United States - District of Columbia
Volume :
7
Issue :
19
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
Available on ORBi :
since 05 November 2021

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