Validation of the analytical procedure for the determination of polyaromatic hydrocarbons in smoke flavourings using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector
[en] High performance liquid chromatography (HPLC) coupled to an ultraviolet (UV), diode array or fluorescence detector (UV/DAD/FLD) has been used to set up an analytical procedure for the quantification of 16 EU priority polyaromatic hydrocarbons (PAHs) in smoke flavourings. The following parameters have been determined for the 16 EU priority PAHs: limit of detection, limit of quantification, precision (repeatability and intermediate precision), recovery and measurement uncertainty, using the concept of accuracy profiles. They were in close agreement with quality criteria described in the Commission Regulation (EC) no. 627/2006 concerning PAHs in smoke flavourings.
Research Center/Unit :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others Chemistry
Author, co-author :
Brasseur, Catherine ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Brose, François ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Pirlot, Alain ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Technologie des denrées alimentaires
Douny, Caroline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Eppe, Gauthier ; Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)
Maghuin-Rogister, Guy ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Language :
English
Title :
Validation of the analytical procedure for the determination of polyaromatic hydrocarbons in smoke flavourings using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector
International Agency for Research on Cancer (IARC) (1983) Polynuclear aromatic compounds. IARC, Lyon, France
United States Environmental Protection Agency (US-EPA) (1984) Polynuclear hydrocarbons, vol 10. US-EPA, Washington, DC
European Commission (2002) Opinion of the Scientific Committee on Food on the risks to human health of polycyclic aromatic hydrocarbons in food, 4 December 2002, SCF/CS/CNTM/PAH/29/Final. Available online at http://europa.eu.int/comm/food/fs/sc/scf/out153_en.pdf
World Health Organization (WHO) (2006) Evaluation of certain food additives. Sixty-fifth report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series, no. 934, 2006. Available online at http://whqlibdoc.who.int/trs/WHO_TRS_934_eng.pdf
The Commission of the European Communities (2005) Commission Regulation (EC) no 208/2005 of 4 February 2005 amending Regulation (EC) no 466/2001 as regards polycyclic aromatic hydrocarbons. Official J EU, vol L 034, pp 3-5 (08/02/2005). Available online at http://eur-lex.europa.eu/LexUriServ/site/en/ oj/2005/l_034/l_03420050208en00030005.pdf
The Commission of the European Communities (2006) Commission Regulation (EC) no 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official J EU, vol L 364, pp 5-24 (20/12/2006). Available online at http://eur-lex.europa.eu/LexUriServ/site/en/oj/2006/l_364/ l_36420061220en00050024.pdf
Simko P (2002) J Chromatogr B 770:3-18
Martinez O, Salmeron J, Guillen MD, Casas C (2004) Food Control 15:457-461
The Commission of the European Communities (2003) Regulation (EC) no 2065/2003 of the European Parliament and of the Council of 10 November 2003 on smoke flavourings used or intended for use in or on foods. Official J EU, vol L 309, pp 1-8 (26/11/2003). Available online at http://europa.eu.int/eur-lex/pri/ en/oj/dat/2003/l_309/l_30920031126en00010008.pdf
The Commission of the European Communities (2006) Commission Regulation (EC) no 627/2006 of 21 April 2006 implementing Regulation (EC) no 2065/2003 of the European Parliament and of the Council as regards quality criteria for validated analytical methods for sampling, identification and characterisation of primary smoke products. Official J EU, vol L 109, pp 3-6 (22/4/2006). Available online at http://eur-lex.europa.eu/LexUriServ/site/en/oj/2006/l_109/ l_10920060422en00030006.pdf
Lodovoci M, Dolara P, Casalini C, Ciappellano S, Testolin G (1995) Food Addit Contam 12:703-713
Simon R, Palme S, Anklam E (2006) J Chromatogr A 1103:307-313
The Commission of the European Communities (2002) Commission Decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Official J EU, vol L 221, pp 8-36 (17/8/2002). Available online at http://eur-lex.europa. eu/LexUriServ/site/en/oj/2002/l_221/l_22120020817en00080036.pdf
Hubert Ph, Nguyen-Huu J-J, Boulanger B, Chapuzet E, Chiap P, Cohen N, Compagnon P-A, Dewé W, Feinberg M, Lallier M, Laurentie M, Mercier N, Muzard G, Nivet C, Valat L (2004) J Pharm Biomed Anal 36:579-586
Hubert Ph, Nguyen-Huu J-J, Boulanger B, Chapuzet E, Chiap P, Cohen N, Compagnon P-A, Dewé W, Feinberg M, Lallier M, Laurentie M, Mercier N, Muzard G, Nivet C, Valat L (2003) STP Pharma Pract 13:101-138
Feinberg M, Laurentie M (2006) Accred Qual Assur: J Qual Compar Reliab Chem Measur 11:3-9
Food and Drug Administration (FDA) (1995) International Conference on Harmonisation. Guideline on validation of analytical procedures: definitions and terminology. Fed Regist 60:11260-11262
The Commission of the European Communities (1996) Council Directive 96/23/EC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products and repealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC. Official J EU, vol L 125, pp 10-32 (23/5/1996). Available online at: http://eur-lex.europa.eu/ LexUriServ/LexUriServ.do?uri=CELEX:31996L0023:EN:HTML
Simon R, Palme S, Anklam E (2006) Food Chem 104:876-887
