pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor

Bouchedja D. N.; Danthine, Sabine; Kar, Tambi; Fickers, Patrick; Sassi H.; Boudjellal A.; Blecker, Christophe; Delvigne, Frank

doi:10.1093/femsle/fny173

Article (Scientific journals)

pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor

Bouchedja D. N.; Danthine, Sabine; Kar, Tambi et al.

2018 • In FEMS Microbiology Letters, 365 (6)

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/233384

DOI
10.1093/femsle/fny173

PubMed
29982388

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Keywords :

Bioreactor, Population analysis; Flow cytometry, Foaming; Emulsion, Anti-foam effect

Abstract :

[en] The oleaginous yeast Yarrowia lipolytica has the ability to use oils and fats as carbon source, making it a promising cell factory for the design of alternative bioprocesses based on renewable substrates. However, such a multiphasic bioreactor design is rather complex and leads to several constraints when considering emulsification of the oil-in-water mixture, foaming and cell growth/physiology on hydrophobic substrate. This study aims to shed light on the effect of pH changes on the physico-chemical properties of the cultivation medium and on cell physiology. It was indeed observed that at a pH value of 6, cell growth rate and intracellular lipid accumulation were optimized. Additionally, foaming was significantly reduced. In order to avoid over foaming in bioreactor, without impairing cell physiology, the use of alternative processes that can only act on the physical structure of culture medium, seems to be an effective alternative to usual chemical anti-foam agents.

Disciplines :

Biotechnology

Author, co-author :

Bouchedja D. N.

Danthine, Sabine ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > SMARTECH

Kar, Tambi

Fickers, Patrick ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Sassi H.

Boudjellal A.

Blecker, Christophe ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > SMARTECH

Delvigne, Frank ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Language :

English

Title :

pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor

Publication date :

04 July 2018

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Oxford University Press, United Kingdom

Volume :

365

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 01 March 2019

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