Unpublished conference/Abstract (Scientific congresses and symposiums)
SFC-MS as a preventive tool for the quality control of potentially adulterated cannabis with synthetic cannabinoids
Jambo, Hugues; Dispas, Amandine; Avohou, Tonakpon Hermane et al.
2018SFC 2018
 

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Abstract :
[en] Recent years have been the stage to a shift in cannabis policies and trends that have impacted cannabis usage and public perception. It has also caught the attention of the pharmaceutical industry and cannabis is increasingly evaluated as a medicine in the treatment of various conditions. On the other hand, there has also been a rise in the development and distribution of synthetic cannabinoids which are synthetic compounds that have the same pharmacological action as the natural cannabinoids found in the plant. They are mostly used as recreational drugs and because their potency and toxicity are not always known, they can lead to severe adverse effects after consumption. The detection of counterfeiting cannabis with synthetic cannabinoids is essential to produce safe cannabis-based medicines. Our aim was to develop a generic supercritical fluid chromatography hyphenated to mass spectrometry (SFC-MS) method that could help in detecting such adulterations using representative synthetic cannabinoids from multiple classes. Method development started with a screening of stationary phases using seven different SFC-dedicated columns. The Torus 1-AA (amino-anthracene) provided the best retention and resolution for the analytes and was selected for the study. Likewise, the mobile phase modifier composition (methanol/water 98:2 v/v) was set after these preliminary tests. The next step performed was the optimization of the method using a design of experiments (DoE) and Bayesian design space (DS) methodology. The temperature, pressure, isocratic and gradient time were selected as parameters for the DoE (central composite design). The separation criterion (S) was set to -0.5 to maximize the separation capacity of the generic method. This Quality by Design (QbD) approach is advantageous as it permits the testing of various conditions within the design space (DS) to achieve a desirable separation since unassessed compounds will probably be encountered during routine analysis. Finally, the quantitative performances were demonstrated by means method validation based on total error approach for the quantification of a selected synthetic cannabinoid in fiber type cannabis plant matrix. Sample preparation was performed with solid-liquid extraction (SLE) followed by filtration and dilution. The acceptance limits were set at ±15% and the β-expectation tolerance limits at 90 % probability level. The results show that the method is valid over the whole dosing range assessed of 2.5 - 7.5% (w/w) with the LOD equal to 14.40 ng/mL. The implementation of this method should be straightforward considering the ease of sample preparation, the use of a fast and green SFC separation and the high specificity and sensitivity achieved with mass spectrometry. Ensuring medicinal cannabis quality is challenging and this work adds an innovative tool that should prove useful in the context of counterfeit drugs tracking.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Chemistry
Author, co-author :
Jambo, Hugues ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Dispas, Amandine  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Avohou, Tonakpon Hermane ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
André, Sébastien ;  Université de Liège - ULiège > Master sc. pharma., à fin.
Hubert, Cédric  ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Lebrun, Pierre ;  Arlenda S.A.
Ziemons, Eric  ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Hubert, Philippe  ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Language :
English
Title :
SFC-MS as a preventive tool for the quality control of potentially adulterated cannabis with synthetic cannabinoids
Publication date :
19 October 2018
Event name :
SFC 2018
Event place :
Strasbourg, France
Event date :
17 - 19 October 2018
Audience :
International
Name of the research project :
FEDER Projet PHARE
Projet Vibra4Fake
Funders :
FEDER - Fonds Européen de Développement Régional [BE]
Région wallonne [BE]
Fonds Léon Fredericq [BE]
Available on ORBi :
since 21 August 2018

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