Poster (Scientific congresses and symposiums)
Quality control of medicinal cannabis: implementation of a generic sfc-ms method to address counterfeiting with synthetic cannabinoids
Jambo, Hugues; Dispas, Amandine; Avohou, Tonakpon Hermane et al.
2018International Symposium DA-PBA 2018
 

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Abstract :
[en] There is a growing interest in using cannabis for medicinal purposes as research shows evidence of its therapeutic properties. However, to be successfully ported in the pharmaceutical field, several aspects such as its quality must be evaluated and ensured. In this context, we address the possibility of counterfeiting of cannabis with synthetic cannabinoids and report the development of a robust method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) that could help in detecting such adulterations. Considering the high number of already available synthetic cannabinoids and the high rate of development of novel structures, we aimed to develop a generic method suitable for the analysis of a large panel of substances using seventeen synthetic cannabinoids from multiple classes as model compounds. Firstly, a suitable column was chosen after a screening phase. The mobile phase (modifier composition) was also set after these preliminary tests. Secondly, a method optimization was carried out using a design of experiments (DoE) and Bayesian design space (DS) methodology that follows ICH Q8 R2 guideline recommendations. This approach is increasingly recommended for the robust optimization of analytical methods. The DoE selected was a four-factor central composite design. Then, according to the goal of adequately analyzing future unknown compounds, the criterion separation S was set to -0.5 to obtain a method with the highest separation capacity. This quality by design (QbD) approach shows flexibility as it permits the testing of various conditions within the DS to tune the separation taking into account that some adaptations might be needed during routine analysis, since it is impossible to predict which compound will be found. Finally, the quantitative performances of the method were demonstrated by means of a validation step based on total error approach for the quantification of a selected synthetic cannabinoid in fiber type cannabis plant matrix. Sample preparation was performed with solid-liquid extraction (SLE) followed by filtration and dilution. The acceptance limits were set at ±15% and the β- expectation tolerance limits at 90 % probability level. The results show that the method is valid over the whole dosing range assessed of 2.5 - 7.5% (w/w) and the LOD equal to 14.40 ng/mL. The implementation of this method should be straightforward considering the ease of sample preparation, the use of a simple modifier composition and the high specificity and sensitivity achieved with mass spectrometry. This work adds an innovative tool to address the challenges of ensuring medicinal cannabis quality and will prove useful in the context of counterfeit drugs tracking.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Jambo, Hugues ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Dispas, Amandine  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Avohou, Tonakpon Hermane ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
André, Sébastien ;  Université de Liège - ULiège > Master sc. pharma., à fin.
Hubert, Cédric  ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Lebrun, Pierre ;  Arlenda S.A.
Ziemons, Eric  ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Hubert, Philippe  ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Language :
English
Title :
Quality control of medicinal cannabis: implementation of a generic sfc-ms method to address counterfeiting with synthetic cannabinoids
Publication date :
2018
Event name :
International Symposium DA-PBA 2018
Event organizer :
KU Leuven
Event place :
Leuven, Belgium
Event date :
9 -12 september 2018
Audience :
International
Funders :
FEDER Projet PHARE
Région wallonne Projet Vibra4Fake
Fonds Léon Fredericq [BE]
Available on ORBi :
since 26 June 2018

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