Reference : Development of an HPTLC method for determination of hypoglycin A in aqueous extracts ...
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Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/212226
Development of an HPTLC method for determination of hypoglycin A in aqueous extracts of seedlings and samaras of Acer species.
English
Habyarimana, Jean Belt Adélite mailto [Université de Liège > Département des sciences fonctionnelles (DSF) > Pharmacologie, pharmacothérapie et toxicologie >]
Baise, Etienne [Université de Liège - ULiège > Department of animal Productions > Biostatistics, Economy and animal selection, Fundamental and Applied Research for Animals & Health (FARAH) > >]
Douny, Caroline mailto [Université de Liège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires >]
Weber, Mireille mailto [Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF) >]
BOEMER, François mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service de génétique >]
De Tullio, Pascal mailto [Université de Liège > Département de pharmacie > Chimie pharmaceutique >]
Franck, Thierry mailto [Université de Liège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Marcillaud-Pitel, C. mailto [Réseau d’EpidémioSurveillance en Pathologie Equine (RESPE) > 14000 Caen - France > > >]
Frederich, Michel mailto [Université de Liège > Département de pharmacie > Pharmacognosie >]
Mouithys-Mickalad, Ange mailto [Université de Liège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Richard, Eric mailto [Normandie Université, UNICAEN, SF 4206 ICORE > LABÉO Frank Duncombe > Caen Cedex 4, France > >]
Scippo, Marie-Louise mailto [Université de Liège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires >]
Votion, Dominique mailto [Université de Liège > > Scientifiques attachés au Doyen (F MV) >]
Gustin, Pascal mailto [Université de Liège > Département des sciences fonctionnelles (DSF) > Pharmacologie, pharmacothérapie et toxicologie >]
9-Jun-2017
No
[en] Hypoglycin A ; HPTLC ; validation ; equine atypical myopathy
[en] Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo, is claimed to be present where AM cases were reported in the US. For unknown reasons, occurrence of this disease has increased. It is important to define environmental key factors that may influence toxicity of samaras from Acer species. In addition, the content of HGA in seedlings needs to be determined since AM outbreaks, during autumn period when the seeds fall but also during spring when seeds are germinating. The present study aims to validate a reliable method using high performance thin layer chromatography for determination and comparison of HGA in samaras and seedlings.
The working range of the method was between 20 μg HGA to 408 μg HGA per ml water, corresponding to 12 - 244 mg/kg fresh weight or 40 - 816 mg/kg dry weight, taking into account of an arbitrary average dry matter content of 30%. Instrumental limit of detection and limit of quantification were of 10 µg HGA/ml and 20 µg HGA/ml water, respectively. Instrumental precision was 4% (RSD on 20 repeated measurements) while instrumental accuracy ranged between 86% and 121% of expected value. The HGA recovery of the analytical method estimated from spiked samaras and seedlings samples ranged between 63 and 103%. The method was applied to 9 samples of samaras from Acer pseudoplatanus, A. platanoides and A. campestre and 5 seedlings samples from A. pseudoplatanus. The results confirm detection of HGA in samaras from A. pseudoplatanus and the absence of detection in samaras of other tested species. They also suggest that detected levels of HGA are highly variable. This confirmed the suitability of the method for HGA detection in samaras or seedling.
Researchers ; Professionals ; Students ; General public ; Others
http://hdl.handle.net/2268/212226
10.1101/148262
Article en attente de soumission au journal
http://biorxiv.org/content/early/2017/06/09/148262

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