Reference : Monitoring of anatabine release by methyl jasmonate elicited BY-2 cells using surface...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/200832
Monitoring of anatabine release by methyl jasmonate elicited BY-2 cells using surface-enhanced Raman scattering
English
De Bleye, Charlotte mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
Dumont, Elodie mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
Dispas, Amandine mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
Hubert, Cédric mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
Sacre, Pierre-Yves mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
Netchacovitch, Lauranne mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
De Muyt, Bénédicte [Université de Liège - ULiège > Département des Sciences de la Vie > Biologie Moléculaire et Biotechnologie Végétales > >]
Kevers, Claire mailto [Université de Liège > Département des sciences de la vie > Département des sciences de la vie >]
Dommes, Jacques mailto [Université de Liège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Hubert, Philippe mailto [Université de Liège > Département de pharmacie > Chimie analytique >]
Ziemons, Eric mailto [Université de Liège > Département de pharmacie > Département de pharmacie >]
Aug-2016
Talanta
Elsevier Science
160
754-760
Yes (verified by ORBi)
International
0039-9140
1873-3573
Amsterdam
The Netherlands
[en] Surface-enhanced Raman scattering ; Monitoring of anatabine release ; BY-2 cells ; Calibration in the matrix ; Gold nanoparticles
[en] A new application of surface-enhanced Raman scattering (SERS) in the field of plant material analysis is proposed in this study. The aim was to monitor the release of anatabine by methyl jasmonate (MeJa) elicited Bright Yellow-2 (BY-2) cells. Gold nanoparticles (AuNps) were used as SERS substrate. The first step was to study the SERS activity of anatabine in a complex matrix comprising the culture medium and BY-2 cells. The second step was the calibration. This one was successfully performed directly in the culture medium in order to take into account the matrix effect, by spiking the medium with different concentrations of anatabine, leading to solutions ranging from 250 to 5000 µg L-1. A univariate analysis was performed, the intensity of a band situated at 1028 cm-1, related to anatabine, was plotted against the anatabine concentration. A linear relationship was observed with a R2 of 0.9951. During the monitoring study, after the MeJa elicitation, samples were collected from the culture medium containing BY-2 cells at 0, 24h, 48h, 72h and 96h and were analyzed using SERS. Finally, the amount of anatabine released in the culture medium was determined using the response function, reaching a plateau after 72h of 82 µg of anatabine released / g of fresh weight (FW) MeJa elicited BY-2 cells.
Centre Interfacultaire de Recherche du Médicament - CIRM
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals
http://hdl.handle.net/2268/200832
10.1016/j.talanta.2016.08.027

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
CDEBLEYE_Talanta_2016.pdfPublisher postprint673.89 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.