Article (Scientific journals)
A fungal biofilm reactor based on metal structured packing improves the quality of a Gla::GFP fusion protein produced by Aspergillus oryzae
Zune, Quentin; Delepierre, Anissa; Gofflot, Sebastien et al.
2015In Applied Microbiology and Biotechnology
Peer Reviewed verified by ORBi
 

Files


Full Text
Author's personal copy AMAB_GlaB.pdf
Publisher postprint (2.1 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Fungal biofilm; Bioreactor; Scale-up
Abstract :
[en] Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state related physiological mechanisms. This work is based on the comparative analysis of classical submerged fermentation with a fungal biofilm reactor for the production of a Gla::GFP fusion protein by Aspergillus oryzae. The biofilm reactor comprises a metal structured packing allowing the attachment of the fungal biomass. Since the production of the target protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, the biofilm mode of culture is expected to enhance the global productivity. Although production of the target protein was enhanced by using the biofilm mode of culture, we also found that fusion protein production is also significant when the submerged mode of culture is used. This result is related to high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2D-gel electrophoresis highlights the preservation of fusion protein integrity produced in biofilm conditions. Two fungal biofilm reactor designs were then investigated further, i.e. with full immersion of the packing or with medium recirculation on the packing, and the scale-up potentialities were evaluated. In this context, it has been shown that full immersion of the metal packing in the liquid medium during cultivation allows for a uniform colonization of the packing by the fungal biomass and leads to a better quality of the fusion protein.
Research center :
Microbial Processes and Interactions
Disciplines :
Biotechnology
Author, co-author :
Zune, Quentin ;  Université de Liège > Chimie et bio-industries > Bio-industries
Delepierre, Anissa ;  Université de Liège > Chimie et bio-industries > Bio-industries
Gofflot, Sebastien;  CRAW > Unit of Products Transformation Technology
Bauwens, Julien ;  Université de Liège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Twizere, Jean-Claude  ;  Université de Liège > Chimie et bio-industries > Biologie cell. et moléc.
Punt, P. J.;  TNO > Microbiology & Systems Biology
Francis, Frédéric  ;  Université de Liège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Bawin, Thomas ;  Université de Liège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Toye, Dominique  ;  Université de Liège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques
Delvigne, Frank  ;  Université de Liège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
A fungal biofilm reactor based on metal structured packing improves the quality of a Gla::GFP fusion protein produced by Aspergillus oryzae
Publication date :
06 May 2015
Journal title :
Applied Microbiology and Biotechnology
ISSN :
0175-7598
eISSN :
1432-0614
Publisher :
Springer Verlag, Berlin, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 06 May 2015

Statistics


Number of views
193 (46 by ULiège)
Number of downloads
393 (35 by ULiège)

Scopus citations®
 
18
Scopus citations®
without self-citations
8
OpenCitations
 
19

Bibliography


Similar publications



Contact ORBi