Reference : Identification of Cytotoxic and Antioxidant Compounds from Allium gramineum Flowers
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/180874
Identification of Cytotoxic and Antioxidant Compounds from Allium gramineum Flowers
English
Mskhiladze, Lasha []
Chincharadze, David []
Tits, Monique mailto [Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie >]
Wauters, Jean-Noël mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
Bellahcene, Akeila mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Castronovo, Vincenzo mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Franck, Thierry mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Frederich, Michel mailto [Université de Liège - ULiège > Département de pharmacie > Pharmacognosie >]
2015
International Journal of Pharmaceutical Sciences and Drug Research
International Journal of Pharmaceutical Sciences and Drug Research
7
78-82
Yes (verified by ORBi)
International
0975-248X
India
[en] The present study evaluates the in vitro anticancer, antiplasmodial and antioxidant activity of the ethanolic crude extract from the flowers of Allium gramineum growing in Georgia and of one flavonol and two steroidal glycosides which were isolated from this plant. The flowers were extracted with ethanol and this total extract was subjected to successive bioguided fractionations to provide glycosides 1-3. Their structures were elucidated on the basis of NMR and ESI-MS spectrometric data in comparison with the existing literature and have been identified as: isorhamnetin-3-O-β-D-glucopyranoside (1), diosgenin-3-O-α-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (Prosapogenin A of dioscin) (2), diosgenin-3-O-α-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)-]-β-D-glucopyranoside (Deltonine) (3). The ethanolic extract has been shown to strongly inhibit the growth of breast adenocarcinoma cell lines, with an IC50 of 4.5 ± 0.7μg/mL for MDAMB-231 and 4.8 ± 0.9μg/mL for MCF-7 cells. The cytotoxic activity was related to 2 and 3 which exhibited potent cytotoxicity, with an IC50 of ± 3μM. Concerning antiplasmodial activities, only weak activities were observed using the ethanolic extract and the two saponins. The flavonoid was almost inactive. Finally, the radical-scavenging activity of the ethanolic extract was tested in presence of ABTS·+ solution. A decrease of the absorbance intensity was observed, with an IC50 value of 22.1 ± 0.6μg/mLwhile trolox, used as Standard drug, showed a pronounced activity (IC50 = 12.7±0.5μM). The glycoside 1 showed the lowest IC50 value of 20.1 ± 0.8μM while both 2 and 3 exhibited very weak radical scavenging activity.
http://hdl.handle.net/2268/180874

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