Article (Scientific journals)
Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1)
Mouithys-Mickalad, Ange; Deby, Ginette; Dogné, Jean-Michel et al.
2004In Biochemical and Biophysical Research Communications, 325 (4), p. 1122-1130
Peer reviewed
 

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Keywords :
Chlamydia pneumoniae; cyclooxygenase-2 enzyme; NADPH-oxidase; COX-2 inhibitors; ROS; electron paramagnetic resonance; chemiluminescence; fluorescence; oxymetry
Abstract :
[en] Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within atherosclerotic lesions. Reactive oxygen species produced by NADPH oxidase were found to trigger the cyclooxygenase-2 expression. The effects of preferential COX-2 inhibitors on ROS produced by Chlamydia-primed human monocytes (THP-1 cells) were evaluated by fluorescence, chemiluminescence, oxymetry, and EPR spin trapping. Fluorescence assays showed an increased production of ROS with Chlamydia versus cells primed by 10(-8) M PMA. COX-2 inhibitors inhibited in a dose-dependent manner the luminol-enhanced CL while ibuprofen and diclofenac increased the chemiluminescence response. By EPR spin trapping, COX-2 inhibitors, ibuprofen, and diclofenac, exhibited a dose-dependent inhibiting effect (10 and 100 muM) on the EPR signal appearance. Our cell model combining EPR, chemiluminescence, and oxymetry appeared relevant to study the modulating effects of preferential COX-2 inhibitors on the cell oxidant activity and chronic inflammatory diseases. (C) 2004 Elsevier Inc. All rights reserved.
Disciplines :
Biochemistry, biophysics & molecular biology
Physics
Pharmacy, pharmacology & toxicology
Author, co-author :
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby, Ginette ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Dogné, Jean-Michel ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
de Leval, X.
Kohnen, Stephan ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Navet, Rachel ;  Université de Liège - ULiège > GIGA - Formation
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Pirotte, Bernard ;  Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique
Lamy, Maurice ;  Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Language :
English
Title :
Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1)
Publication date :
2004
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
325
Issue :
4
Pages :
1122-1130
Peer reviewed :
Peer reviewed
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