Three factors that modulate the activity of class D β-lactamases and interfere with the post-translational carboxylation of Lys 70

Vercheval, Lionel; Di Paolo, Alexandre; Borel, Franck; Ferrer, Jean-Luc; Sauvage, Eric; Matagne, André; Frère, Jean-Marie; Charlier, Paulette; Galleni, Moreno; Kerff, Frédéric

doi:10.1042/BJ20101122

Article (Scientific journals)

Three factors that modulate the activity of class D β-lactamases and interfere with the post-translational carboxylation of Lys 70

Vercheval, Lionel; Di Paolo, Alexandre; Borel, Franck et al.

2010 • In Biochemical Journal, 432 (3), p. 495-504

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https://hdl.handle.net/2268/74943

DOI
10.1042/BJ20101122

PubMed
21108605

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Keywords :

beta-lactamase; chloride inhibition; hydrophobic core; Lysine carboxylatin; post-translational modification

Abstract :

[en] Lys-70 carboxylation in the active site of class D β lactamases is essential for their activity. Structural, kinetic and affinity studies show that this post-translational modification can be affected by the presence of a poor substrate such as moxalactam but also by the V117T substitution. Val-117 is a strictly conserved hydrophobic residue located in the active site. In addition, inhibition of class D β lactamases by chloride ions is due to a competition between the side chain carboxylate of the modified Lys 70 and chloride ions. Determination of the individual kinetic constants shows that the deacylation of the acyl-enzyme is the rate limiting step for the wild type OXA 10 β lactamase.

Research Center/Unit :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Vercheval, Lionel ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Di Paolo, Alexandre ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Borel, Franck

Ferrer, Jean-Luc

Sauvage, Eric ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Matagne, André ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie et repliement des protéines

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Charlier, Paulette ; Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques

Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Kerff, Frédéric ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Three factors that modulate the activity of class D β-lactamases and interfere with the post-translational carboxylation of Lys 70

Publication date :

25 November 2010

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, London, United Kingdom

Volume :

432

Issue :

Pages :

495-504

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 03 November 2010

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