The Bimodular G57-V577 Polypeptide Chain of the Class B Penicillin-Binding Protein 3 of Escherichia Coli Catalyzes Peptide Bond Formation from Thiolesters and Does Not Catalyze Glycan Chain Polymerization from the Lipid II Intermediate
[en] Because the specificity profile of the membrane anchor-free G57-V577 penicillin-binding protein 3 (PBP3) of Escherichia coli for a large series of beta-lactam antibiotics is similar to that of the full-size membrane-bound PBP, the truncated PBP is expected to adopt the native folded conformation. The truncated PBP3 functions as a thiolesterase. In aqueous media and in the presence of millimolar concentrations of a properly structured amino compound, it catalyzes the aminolysis of the thiolester until completion, suggesting that the penicillin-binding module of PBP3 is designed to catalyze transpeptidation reactions. In contrast, the truncated PBP3 is devoid of glycan polymerization activity on the E. coli lipid II intermediate, suggesting that the non-penicillin-binding module of PBP3 is not a transglycosylase.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Adam, Maggy; Université de Liège - ULiège > Institut de Chimie > Centre d'ingénierie des protéines
Fraipont, Claudine ; Université de Liège - ULiège > Institut de Chimie > Centre d'ingénierie des protéines
Rhazi, Noureddine ; Université de Liège - ULiège > Institut de Chimie > Centre d'ingénierie des protéines
Nguyen-Disteche, Martine ; Université de Liège - ULiège > Institut de Chimie > Centre d'ingénierie des protéines
Lakaye, Bernard ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénirie des Protéines > Laboratoire d'Enzymologie
Frère, Jean-Marie ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénirie des Protéines > Laboratoire d'Enzymologie
Devreese, Bart; Rijksunversiteit-Gent > Laboratorium voor Eiwitbiochemie en Eiwitengineering
Van Beeumen, Jozef; Rijksunversiteit-Gent > Laboratorium voor Eiwitbiochemie en Eiwitengineering
van Heijenoort, Yvelyne; Université Paris-Sud 11 > Laboratoire de Biochimie Moléculaire et Cellulaire
van Heijenoort, Jean; Université Paris-Sud 11 > Laboratoire de Biochimie Moléculaire et Cellulaire
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Institut de Chimie > Centre d'ingénierie des protéines
Language :
English
Title :
The Bimodular G57-V577 Polypeptide Chain of the Class B Penicillin-Binding Protein 3 of Escherichia Coli Catalyzes Peptide Bond Formation from Thiolesters and Does Not Catalyze Glycan Chain Polymerization from the Lipid II Intermediate
Publication date :
October 1997
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
179
Issue :
19
Pages :
6005-6009
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRFC - Fonds de la Recherche Fondamentale Collective
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