Article (Scientific journals)
Housekeeping Genes as Internal Standards: Use and Limits
Thellin, Olivier; Zorzi, Willy; Lakaye, Bernard et al.
1999In Journal of Biotechnology, 75 (2-3), p. 291-5
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Keywords :
Amplification; RNA; PCR
Abstract :
[en] Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins, cyclophilin, hypoxantine phsophoribosyltransferase (HRPT), L32. 28S, and 18S rRNAs are also used as internal standards. In this paper, it is recalled that the commonly used internal standards can quantitatively vary in response to various factors. Possible variations are illustrated using three experimental examples. Preferred types of internal standards are then proposed for each of these samples and thereafter the general procedure concerning the choice of an internal standard and the way to manage its uses are discussed.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Thellin, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Willy ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Lakaye, Bernard ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
De Borman, B.
Coumans, Bernard ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Hennen, Georges ;  Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Grisar, Thierry ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Igout, Ahmed ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Language :
English
Title :
Housekeeping Genes as Internal Standards: Use and Limits
Publication date :
1999
Journal title :
Journal of Biotechnology
ISSN :
0168-1656
eISSN :
1873-4863
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
75
Issue :
2-3
Pages :
291-5
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 November 2010

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