Article (Scientific journals)
Proteome analysis of the bovine milk fat globule: Enhancement of membrane purification
Vanderghem, Caroline; Blecker, Christophe; Danthine, Sabine et al.
2008In International Dairy Journal, 18 (9), p. 885-893
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Abstract :
[en] A simple and rapid procedure was cl developed for the extraction of the milk fat globule membrane from milk removes the large majority of the skim milk proteins for proteome analysis. In order to improve the extraction and the solubilization of the hydrophobic membrane proteins for subsequent two-dimensional gel electrophoresis, four detergents (3-[(3-cholamidopropyl)dimethylammoniol-1-propanesulfonate, amidosulfobetaine-14, sodium lauroyl sarcosinate and sodium deoxycholate) were tested in the sample preparation, associated with a sonication step. Zwitterionic detergents were shown to be efficient in recovering integral and peripheral proteins from membrane material. Spots were identified by matrix-assisted laser desorption/ionization tandem-time-of-flight (MALDI-TOF/TOF). The advantages of MALDI-TOF/TOF (speed, easiness of analysis, good sensitivity and high mass accuracy) were demonstrated on the milk fat globule membrane proteome investigation. Identified proteins are implicated in a wide range of functions including fat secretion and transport, protein trafficking and regulation. (C) 2008 Elsevier Ltd. All rights reserved.
Disciplines :
Agriculture & agronomy
Food science
Author, co-author :
Vanderghem, Caroline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Blecker, Christophe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Danthine, Sabine  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Deroanne, Claude ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Haubruge, Eric  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Guillonneau, Francois
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Francis, Frédéric  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Language :
English
Title :
Proteome analysis of the bovine milk fat globule: Enhancement of membrane purification
Publication date :
2008
Journal title :
International Dairy Journal
ISSN :
0958-6946
Publisher :
Elsevier Science
Volume :
18
Issue :
9
Pages :
885-893
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 December 2009

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