Unveiling the influence of a probiotic combination of Heyndrickxia coagulans and Lacticaseibacillus casei on healthy human gut microbiota using the TripleSHIME® system.
Bacillus coagulans; Colonic microbiota; Gastrointestinal simulation; Gut fermentation; Lactobacillus casei; Probiotic cocktail; Gastrointestinal; Human gut microbiota; Probiotics; Microbiology
Abstract :
[en] Probiotics are host-friendly microorganisms that can have important health benefits in the human gut microbiota as dietary supplements. Maintaining a healthy gut microbial balance relies on the intricate interplay among the intestinal microbiota, metabolic activities, and the host's immune response. This study aims to explore if a mixture of Heyndrickxia coagulans [ATB-BCS-042] and Lacticaseibacillus casei [THT-030-401] promotes in vitro this balance in representative gut microbiota from healthy individuals using the Triple-SHIME® (Simulation of the Human Intestinal Microbial Ecosystem). Metataxonomic analysis of the intestinal microbes revealed that the probiotic mix was not causing important disruptions in the biodiversity or microbial composition of the three simulated microbiota. However, some targeted populations analyzed by qPCR were found to be disrupted at the end of the probiotic treatment or after one week of washout. Populations such as Cluster IV, Cluster XVIa, and Roseburia spp., were increased indicating a potential gut health-promoting butyrogenic effect of the probiotic supplementation. In two of the systems, bifidogenic effects were observed, while in the third, the treatment caused a decrease in bifidobacteria. For the health-detrimental biomarker Escherichia-Shigella, a mild decrease in all systems was observed in the proximal colon sections, but these genera were highly increased in the distal colon sections. By the end of the washout, Bacteroides-Prevotella was found consistently boosted, which could have inflammatory consequences in the intestinal context. Although the probiotics had minimal influence on most quantified metabolites, ammonia consistently decreased after one week of daily probiotic supplementation. In reporter gene assays, aryl hydrocarbon receptor (AhR) activation was favored by the metabolic output obtained from post-treatment periods. Exposure of a human intestinal cell model to fermentation supernatant obtained after probiotic supplementation induced a trend to decrease the mRNA expression of immunomodulatory cytokines (IL-6, IL-8). Overall, with some exceptions, a positive impact of H. coagulans and L. casei probiotic mix was observed in the three parallel experiments, despite inter-individual differences. This study might serve as an in vitro pipeline for the impact assessment of probiotic combinations on the human gut microbiota.
Disciplines :
Food science Microbiology
Author, co-author :
Goya-Jorge, Elizabeth ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Gonza Quito, Irma Elizabeth ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Bondue, Pauline; Laboratory of Food Quality Management, Department of Food Sciences, FARAH - Veterinary Public Health, University of Liège, Liège 4000, Belgium. Electronic address: pauline.bondue@ortis.com
Druart, Germain; Lacto Research sprl., Rue Herman Meganck 21, Gembloux-les Isnes 5032, Belgium. Electronic address: germain.druart@tht.be
Al-Chihab, Mohamed; Lacto Research sprl., Rue Herman Meganck 21, Gembloux-les Isnes 5032, Belgium. Electronic address: m.al-chihab@lactoresearch.be
Boutaleb, Samiha ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA)
Douny, Caroline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Taminiau, Bernard ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Thonart, Philippe; Lacto Research sprl., Rue Herman Meganck 21, Gembloux-les Isnes 5032, Belgium. Electronic address: p.thonart@artechno.be
Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire
Language :
English
Title :
Unveiling the influence of a probiotic combination of Heyndrickxia coagulans and Lacticaseibacillus casei on healthy human gut microbiota using the TripleSHIME® system.
Financial support for this research work was obtained from the Walloon Region, Belgium in the context of GutTransit Project (\u201CDevelopment of innovative nutritional ingredients to stimulate transit and improve intestinal health\u201D) with Grant Agreement No 8225. We are grateful to Prof. Benoit Cudennec (Universit\u00E9 de Lille) for kindly providing the Caco-2 cell line (ATCC) at a low passage number allowing us to optimize our protocol for long-term cell culture inserts and to Dr. C\u00E9line Antoine for designing the cartoon images of the SHIME system. The graphical abstract was created with BioRender.com.
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