Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector.
[en] This work reports the characterization of the lipidic fraction of seven species of marine organisms gathered along the shoreline of the Po Delta Park of Emilia-Romagna Region (Italy) and of the north Adriatic Sea. Two species of oysters (Crassostrea gigas and Ostrea edulis), two species of clams (Chamelea gallina and Ruditapes philippinarum), one species of mussel (Mytilus galloprovincialis), one species of macroalgae (Ulva rigida), and one species of spiny dogfish (Squalus acanthias) were analyzed to characterize their fatty acids profile and related nutritional value. The lipid fraction was simultaneously extracted and transesterified into fatty acid methyl esters (FAMEs) by using a recently developed one-step microwave-assisted extraction/derivatization (MAED) method. The obtained FAMEs extract was analyzed by a rapid comprehensive multidimensional gas chromatography (GC × GC) method (30 min). The system was equipped with a reverse set of columns (polar × non-polar) connected through a reversed fill/flush flow modulator. The GC × GC system was coupled with a flame-ionization detector (FID) for both qualitative and quantitative purposes. The MAED- GC × GC-FID methodology was suitable in the context of samples containing high percentages of omega-3 PUFA. A total of 82 FAMEs were tentatively identified using standards, literature data, and the two-dimensional plot location. FAME profiles obtained with the proposed approach were comparable with reference methods (AOCS Ce 2b-11), showing no significant differences. Moreover, to determine the food nutritional value of the samples investigated, the most common nutritional indices (index of atherogenicity, index thrombogenicity, hypocholesterolemic/hypercholesterolemic ratio, health-promoting index, unsaturation index, and the fish lipid quality index) were calculated from FAME profiles. Among the samples investigated, Squalus acanthias presented the best nutritional score, while Ruditapes philippinarum had the worst score in 3 out of 6 indices.
Cescon, Mirco; Department of Chemical, Pharmaceutical, and Agricultural Sciences, Via Luigi Borsari 46, University of Ferrara, 44121 Ferrara, Italy
Giacoppo, Giulia; Department of Chemical, Pharmaceutical, and Agricultural Sciences, Via Luigi Borsari 46, University of Ferrara, 44121 Ferrara, Italy
Costa, Valentina; Department of Environmental and Prevention Sciences, University of Ferrara, Via L. Borsari 46, 44121 Ferrara, Italy
Purcaro, Giorgia ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Spadafora, Natasha Damiana; Department of Chemical, Pharmaceutical, and Agricultural Sciences, Via Luigi Borsari 46, University of Ferrara, 44121 Ferrara, Italy
Cordero, Chiara; Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, I-10125 Torino, Italy
Pasti, Luisa; Department of Environmental and Prevention Sciences, University of Ferrara, Via L. Borsari 46, 44121 Ferrara, Italy. Electronic address: luisa.pasti@unife.it
Cavazzini, Alberto; Department of Chemical, Pharmaceutical, and Agricultural Sciences, Via Luigi Borsari 46, University of Ferrara, 44121 Ferrara, Italy
Beccaria, Marco ; Université de Liège - ULiège > Molecular Systems (MolSys)
Language :
English
Title :
Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector.
Publication date :
01 April 2024
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
The authors would like to thank the National Recovery and Resilience Plan (NRRP), Mission 04 Component 2 Investment 1.5 – NextGenerationEU, Call for tender n. 3277 dated 30/12/2021; Award Number: 0001052 dated 23/06/2022. The authors acknowledge Naturelis Srl for having provided bivalve samples. Natasha Damiana Spadafora benefits from the funding of the program PON “Research and Innovation” 2014–2020 (PON R&I), Action IV.6 “Contratti di ricerca su tematiche Green.” The authors would like to thank Milestone S.r.L. Shimadzu Corporation, and SepSolve Analytical for their support.
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