A New, Highly Sensitive Method for the Detection and Quantification of Penicillin-Binding Proteins

[en] A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

Research Center/Unit :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Lakaye, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique

Lepage, Sophie

Jamin, M.

Thamm, Iris ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie

Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Frère, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie

Language :

English

Title :

A New, Highly Sensitive Method for the Detection and Quantification of Penicillin-Binding Proteins

Publication date :

01 April 1993

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, United Kingdom

Volume :

291

Issue :

(Pt 1)

Pages :

19-21

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 November 2009

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