Article (Scientific journals)
Microwave-Assisted Saponification Method Followed by Solid-Phase Extraction for the Characterization of Sterols and Dialkyl Ketones in Fats
Mascrez, Steven; Danthine, Sabine; Purcaro, Giorgia
2021In Foods, 10, p. 445
Peer Reviewed verified by ORBi
 

Files


Full Text
81_Foods_2021_10 445.pdf
Publisher postprint (1.5 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
dialkylketones;; sterols; microwave-assisted saponification; solid-phase extraction; chemical interesterification; gas chromatography
Abstract :
[en] Unlike other fields, the methods routinely applied for fats and oils are still tied to traditional, time- and solvent-consuming procedures, such as saponification, column chromatography and thin-layer chromatography. In this paper, microwave-assisted saponification followed by a labmade solid-phase extraction was optimized for the characterization of either dialkyl ketones (DAK)or sterols or both simultaneously. The instrumental determination was performed by gas chromatography-flame ionization detector (GC-FID) for quantification and gas chromatography-mass spectrometry (GC-MS) for confirmation purposes. The proposed method showed good recoveries (>80%) and limit of quantification (0.04–0.07 μg/g for the 4 DAK and of 0.07 μg/g for α-cholestanol). Repeatabilities (n = 3) were below 15% for DAKs and generally lower than 6% for sterols. Accuracy on the entire sterol profile was confirmed in comparison to the International Olive Council reference method. The method was finally applied to real-world samples before and after chemical interesterification.
Disciplines :
Food science
Chemistry
Author, co-author :
Mascrez, Steven ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Danthine, Sabine  ;  Université de Liège - ULiège > Département GxABT > SMARTECH
Purcaro, Giorgia  ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Language :
English
Title :
Microwave-Assisted Saponification Method Followed by Solid-Phase Extraction for the Characterization of Sterols and Dialkyl Ketones in Fats
Publication date :
2021
Journal title :
Foods
eISSN :
2304-8158
Publisher :
MDPI AG, Switzerland
Special issue title :
Characterization and Detection of Lipid Substances in Food
Volume :
10
Pages :
445
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 February 2021

Statistics


Number of views
68 (12 by ULiège)
Number of downloads
69 (12 by ULiège)

Scopus citations®
 
5
Scopus citations®
without self-citations
3
OpenCitations
 
4

Bibliography


Similar publications



Contact ORBi