dialkylketones;; sterols; microwave-assisted saponification; solid-phase extraction; chemical interesterification; gas chromatography
Abstract :
[en] Unlike other fields, the methods routinely applied for fats and oils are still tied to traditional,
time- and solvent-consuming procedures, such as saponification, column chromatography
and thin-layer chromatography. In this paper, microwave-assisted saponification followed by a labmade solid-phase extraction was optimized for the characterization of either dialkyl ketones (DAK)or sterols or both simultaneously. The instrumental determination was performed by gas chromatography-flame ionization detector (GC-FID) for quantification and gas chromatography-mass spectrometry (GC-MS) for confirmation purposes. The proposed method showed good recoveries (>80%) and limit of quantification (0.04–0.07 μg/g for the 4 DAK and of 0.07 μg/g for α-cholestanol). Repeatabilities (n = 3) were below 15% for DAKs and generally lower than 6% for sterols. Accuracy on the entire sterol profile was confirmed in comparison to the International Olive Council reference method. The method was finally applied to real-world samples before and after chemical interesterification.
Disciplines :
Food science Chemistry
Author, co-author :
Mascrez, Steven ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Danthine, Sabine ; Université de Liège - ULiège > Département GxABT > SMARTECH
Purcaro, Giorgia ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Language :
English
Title :
Microwave-Assisted Saponification Method Followed by Solid-Phase Extraction for the Characterization of Sterols and Dialkyl Ketones in Fats
Publication date :
2021
Journal title :
Foods
eISSN :
2304-8158
Publisher :
MDPI AG, Switzerland
Special issue title :
Characterization and Detection of Lipid Substances in Food
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