Article (Scientific journals)
Dibenzoylthiamine has powerful antioxidant and anti-inflammatory properties in cultured cells and in mouse models of stress and neurodegeneration
Sambon, Margaux; Gorlova, Anna; Demelenne, Alice et al.
2020In Biomedicines, 8, p. 361
Peer Reviewed verified by ORBi
 

Files


Full Text
Sambon_biomedicines-08-00361-v2.pdf
Publisher postprint (6.49 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
thiamine; oxidative stress; inflammation; neurodegeneration; animal model; amyotrophic lateral sclerosis; paraquat; lipopolysaccharide; benfotiamine; sulbutiamine; dibenzoylthiamine
Abstract :
[en] Thiamine precursors, the most studied being benfotiamine (BFT), have protective effects in mouse models of neurodegenerative diseases. BFT decreased oxidative stress and inflammation, two major characteristics of neurodegenerative diseases, in a neuroblastoma cell line (Neuro2a) and an immortalized brain microglial cell line (BV2). Here, we tested the potential antioxidant and anti-inflammatory effects of the hitherto unexplored derivative O,S-dibenzoylthiamine (DBT) in these two cell lines. We show that DBT protects Neuro2a cells against paraquat (PQ) toxicity by counteracting oxidative stress at low concentrations and increases the synthesis of reduced glutathione and NADPH in a Nrf2-independent manner. In BV2 cells activated by lipopolysaccharides (LPS), DBT significantly decreased inflammation by suppressing translocation of NF-κB to the nucleus. Our results also demonstrate the superiority of DBT over thiamine and other thiamine precursors, including BFT, in all of the in vitro models. Finally, we show that the chronic administration of DBT arrested motor dysfunction in FUS transgenic mice, a model of amyotrophic lateral sclerosis, and it reduced depressive-like behavior in a mouse model of ultrasound-induced stress in which it normalized oxidative stress marker levels in the brain. Together, our data suggest that DBT may have therapeutic potential for brain pathology associated with oxidative stress and inflammation by novel, coenzyme-independent mechanisms.
Research Center/Unit :
Giga-Neurosciences - ULiège
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sambon, Margaux ;  Université de Liège - ULiège > GIGA
Gorlova, Anna
Demelenne, Alice ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Alhama-Riba, Judit
Coumans, Bernard ;  Université de Liège - ULiège > Stem Cells-Molecular Regulation of Neurogenesis
Lakaye, Bernard ;  Université de Liège - ULiège > Stem Cells-Molecular Regulation of Neurogenesis
Wins, Pierre
Fillet, Marianne  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Anthony, Daniel C.
Strekalova, Tatyana
Bettendorff, Lucien  ;  Université de Liège - ULiège > Neurosciences-Neurophysiology
Language :
English
Title :
Dibenzoylthiamine has powerful antioxidant and anti-inflammatory properties in cultured cells and in mouse models of stress and neurodegeneration
Publication date :
2020
Journal title :
Biomedicines
eISSN :
2227-9059
Publisher :
MDPI AG, Switzerland
Volume :
8
Pages :
361
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
“5–100” Russian Research Excellence Program
Funders :
Fonds Léon Fredericq
Fonds Léon Fredericq
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
Available on ORBi :
since 16 October 2020

Statistics


Number of views
111 (17 by ULiège)
Number of downloads
65 (11 by ULiège)

Scopus citations®
 
24
Scopus citations®
without self-citations
14
OpenCitations
 
10
OpenAlex citations
 
26

Bibliography


Similar publications



Contact ORBi