Poster (Scientific congresses and symposiums)
Comparison between swabbing devices in order to analyze the microbial flora found on surfaces of community kitchens by classical microbiology and 16S rRNA amplicon sequencing
Krings, Simone; Crevecoeur, Sébastien; Rodriguez Diaz, Cristina et al.
2017FEMS2017 - 7th Congress of European Microbiologists
 

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Keywords :
Food Microbiology; Surfaces; 16S rRNA gene sequencing
Abstract :
[en] Background The work in community kitchens involves several manual steps which justifies accurate control of surfaces to prevent foodborne illnesses. Different sampling methods have been proposed to isolate bacteria from surfaces. However, recovery efficiencies of these methods are very different and not always comparable. Objectives The aim of this study is to evaluate different sampling methods to detect the presence of pathogenic bacteria on surfaces in contact with foods. The recovery efficiency was examined by classical microbiology an culture-independent metagenomic analysis. Methods To mimic surfaces which can be found in community kitchens, sterile polypropylene stainless steel surfaces and cutting boards were spiked with serial dilutions of Escherichia coli, Salmonella enterica and Bacillus cereus. They were left to dry for 1-2 hours before collection of the surviving bacteria with different moistened swabs, including commercial swabs (sponges-HS10NB2G, Sponge-Sticks-SSL100; 3M) and cotton and gauze pads. Different solutions were tested to moisten the swabs (physiological water (0,85% NaCl), Maximum Recovery Diluent and quarter-strength Ringer solution). Bacterial population analysis was performed by classical methods and metagenomics. Conclusions Important differences on bacterial survival were observed depending on their nature and the solution used to collect them from the surfaces. Maximum Recovery Diluent and cotton pads showed the best results in relation with bacteria recovery and required technical skills. Metagenomic analysis showed a higher detection sensibility than classical microbiology. However, preliminary results showed that both methods are necessary to improve the detection of pathogens in surfaces.
Disciplines :
Microbiology
Author, co-author :
Krings, Simone ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Crevecoeur, Sébastien ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Rodriguez Diaz, Cristina ;  Université de Liège - ULiège > R&D Direction : Chercheurs ULg en mobilité
Fall, Papa Abdoulaye;  Genalyse Partner S.A.
Taminiau, Bernard  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Daube, Georges  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Language :
English
Title :
Comparison between swabbing devices in order to analyze the microbial flora found on surfaces of community kitchens by classical microbiology and 16S rRNA amplicon sequencing
Alternative titles :
[fr] Comparaison entre différents écouvillons pour analyser les flores microbiennes trouvées sur des surfaces en cuisines de collectivité par microbiologie classique et métagénétique
Publication date :
11 July 2017
Event name :
FEMS2017 - 7th Congress of European Microbiologists
Event organizer :
FEMS - Federation of European Microbiological Societies
Event place :
Valencia, Spain
Event date :
9 - 13 July 2017
Audience :
International
Available on ORBi :
since 19 September 2018

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