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In vitro evaluation of the competing effect of Carnobacterium maltaromaticum isolated from vacuum-packed beef with long shelf-life against three major food pathogens
Didimo Imazaki, Pedro Henrique; Maia Danielski, Gabriela; Daube, Georges et al.
20174th FARAH Day
 

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Keywords :
biopreservation; Carnobacterium maltaromaticum; beef
Abstract :
[en] Foodborne disease outbreaks are one of the leading causes of infections, hospitalisations and deaths provoked by pathogenic bacteria. Biopreservation is a hurdle which consists of the use of controlled microorganisms or its metabolites to preserve food and extend its shelf-life. Carnobacteria have been studied for their bioprotective properties since they can inhibit pathogenic and spoilage microorganisms. In this way, this study aimed to evaluate in vitro the bioprotective potential of Carnobacterium maltaromaticum (CM) isolated from vacuum-packed (VP) beef against Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Typhimurium. Three CM strains isolated from VP beef with long shelf-life at a temperature near the freezing point were selected. The antimicrobial effect of CM against the pathogenic bacteria cited above was evaluated in co-cultures with or without EDTA at different temperatures. The antimicrobial effect of the cell-free supernatant of CM was also evaluated. The results indicate that the selected strains have an antilisterial activity, which is optimised at low temperatures. Moreover, when the strains were combined with EDTA, it was observed a slight inhibition of gram-negative bacteria (GNB). The inhibition with the EDTA treatment was possibly due to the capacity of this compound to destabilise the outer membrane of GNB, allowing CM to act against these bacteria. Finally, the evaluation of the antimicrobial activity of cell-free supernatant of CM did not highlight any inhibition effect against the tested pathogens. Thus, the antimicrobial activity of these CM strains is not related to bacteriocin production under the studied conditions.
Disciplines :
Food science
Author, co-author :
Didimo Imazaki, Pedro Henrique ;  Université de Liège - ULiège > Doct. sc. vété. (Bologne)
Maia Danielski, Gabriela
Daube, Georges  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Ernlund Freitas de Macedo, Renata
Clinquart, Antoine ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Technologie des denrées alimentaires
Language :
English
Title :
In vitro evaluation of the competing effect of Carnobacterium maltaromaticum isolated from vacuum-packed beef with long shelf-life against three major food pathogens
Publication date :
13 October 2017
Event name :
4th FARAH Day
Event organizer :
FARAH - Fundamental and Applied Research for Animals & Health
Event place :
Liège, Belgium
Event date :
le 13 octobre 2017
Funders :
General Operational Directorate of Agriculture, Natural Resources and Environment (DGARNE) of the Walloon Region (Belgium) [project D31-1275 (CONSBBB)]
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since 08 April 2018

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