[en] Living in a microbe-rich environment reduces the risk of developing asthma. Exposure of humans or mice to unmethylated CpG DNA (CpG) from bacteria reproduces these protective effects, suggesting a major contribution of CpG to microbe-induced asthma resistance. However, how CpG confers protection remains elusive. We found that exposure to CpG expanded regulatory lung interstitial macrophages (IMs) from monocytes infiltrating the lung or mobilized from the spleen. Trafficking of IM precursors to the lung was independent of CCR2, a chemokine receptor required for monocyte mobilization from the bone marrow. Using a mouse model of allergic airway inflammation, we found that adoptive transfer of IMs isolated from CpG-treated mice recapitulated the protective effects of CpG when administered before allergen sensitization or challenge. IM-mediated protection was dependent on IL-10, given that Il10-/- CpG-induced IMs lacked regulatory effects. Thus, the expansion of regulatory lung IMs upon exposure to CpG might underlie the reduced risk of asthma development associated with a microbe-rich environment.
Research Center/Unit :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sabatel, Catherine ✱; Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Radermecker, Coraline ✱; Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Fievez, Laurence ✱; Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Paulissen, Geneviève ; Université de Liège > Département des sciences cliniques > Hépato-gastroentérologie
Chakarov, Svetoslav; Singapore Immunology Network, Agency for Science, Technology, and Research, Singapore 138648, Singapore
Fernandes, Claudia; Université de Liège - ULiège > Laboratory of Cellular and Molecular Immunology, GIGA Research
Olivier, Sabine; Université de Liège - ULiège > Laboratory of Cellular and Molecular Immunology, GIGA Research
Toussaint, Marie; Airway Disease Infection Section, National Heart and Lung Institute, Imperial College London, London W2 1PG, UK
Pirottin, Dimitri ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Xiao, Xue ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Quatresooz, Pascale ; Université de Liège > Département des sciences biomédicales et précliniques > Histologie
Sirard, Jean-Claude; University of Lille,CHU Lille, Institut Pasteur de Lille, France > INSERM U1019, CNRSUMR8204, Center for Infection and Immunity of Lille
Cataldo, Didier ; Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Gillet, Laurent ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Bouabe, Hicham; Babraham Institute, Cambridge CB22 3AT, UK > Laboratory of Lymphocyte Signalling and Development
Desmet, Christophe ; Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Ginhoux, Florent; Singapore Immunology Network, Agency for Science, Technology, and Research, Singapore 138648, Singapore
Marichal, Thomas ✱; Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Bureau, Fabrice ✱; Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
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