Article (Scientific journals)
The transcription factor ERG recruits CCR4-NOT to control mRNA decay and mitotic progression.
Rambout, Xavier; Detiffe, Cecile; Bruyr, Jonathan et al.
2016In Nature Structural and Molecular Biology
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Keywords :
RNA; Decay; deadenylation
Abstract :
[en] Control of mRNA levels, a fundamental aspect in the regulation of gene expression, is achieved through a balance between mRNA synthesis and decay. E26-related gene (Erg) proteins are canonical transcription factors whose previously described functions are confined to the control of mRNA synthesis. Here, we report that ERG also regulates gene expression by affecting mRNA stability and identify the molecular mechanisms underlying this function in human cells. ERG is recruited to mRNAs via interaction with the RNA-binding protein RBPMS, and it promotes mRNA decay by binding CNOT2, a component of the CCR4-NOT deadenylation complex. Transcriptome-wide mRNA stability analysis revealed that ERG controls the degradation of a subset of mRNAs highly connected to Aurora signaling, whose decay during S phase is necessary for mitotic progression. Our data indicate that control of gene expression by mammalian transcription factors may follow a more complex scheme than previously anticipated, integrating mRNA synthesis and degradation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rambout, Xavier ;  Université de Liège > GIGA-Research
Detiffe, Cecile
Bruyr, Jonathan
Mariavelle, Emeline ;  Université de Liège - ULiège > Molecular Biology of Diseases-Gene Expression & Cancer
Cherkaoui, Majid
Brohee, Sylvain
Demoitie, Pauline
Lebrun, Marielle  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Virologie et immunologie
Soin, Romuald
Lesage, Bart
Guedri, Katia ;  Université de Liège > Département des sciences de la vie > Génétique et biologie moléculaires animales
Beullens, Monique
Bollen, Mathieu
Farazi, Thalia A.
Kettmann, Richard ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biologie cell. et moléc.
Struman, Ingrid  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Hill, David E.
Vidal, Marc
Kruys, Veronique
Simonis, Nicolas
Twizere, Jean-Claude  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biologie cell. et moléc.
Dequiedt, Franck  ;  Université de Liège > GIGA-Research
More authors (12 more) Less
Language :
English
Title :
The transcription factor ERG recruits CCR4-NOT to control mRNA decay and mitotic progression.
Publication date :
2016
Journal title :
Nature Structural and Molecular Biology
ISSN :
1545-9993
eISSN :
1545-9985
Publisher :
Nature Publishing Group
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 June 2016

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