Reference : Unusual Amino Acids and Monofluoroacetate from Dichapetalum michelsonii (Umutambasha)...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/159005
Unusual Amino Acids and Monofluoroacetate from Dichapetalum michelsonii (Umutambasha), a Toxic Plant from Rwanda
English
Esters, Virginie mailto [Université de Liège - ULiège > Département de pharmacie > Pharmacognosie >]
Karangwa, Charles [Laboratory of Pharmacology and Toxicology, > Department of Pharmacy > Faculty of Medicine > National University of Rwanda, Butare, Rwanda >]
Tits, Monique mailto [Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie >]
Francotte, Pierre mailto [Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique >]
Pirotte, Bernard mailto [Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique >]
Servais, Anne-Catherine mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Crommen, Jacques mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
Robbrecht, Elmar [Department of Vascular Plants (Spermatophyta-Pteridophyta) > National Botanic Garden of Belgium > Meise, Belgium > >]
MINET, Arlette [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Grisar, Thierry mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Angenot, Luc mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
Frederich, Michel mailto [Université de Liège - ULiège > Département de pharmacie > Pharmacognosie >]
Mar-2013
Planta Medica
Georg Thieme Verlag Stuttgart
79
334-337
Yes (verified by ORBi)
International
0032-0943
New York
NY
[en] Dichapetalum michelsonii ; Dichapetalaceae ; Umutambasha ; sodium monofluoroacetate ; 2,7‐diaminooctan‐1,8‐dioic acid ; Magnistipula butayei
[en] In the course of our investigations on Umutambasha in order to identify its convulsant principles, small quantities of monofluoroacetate were observed in stem bark, leaves, and fruits of this plant newly identified as Dichapetalum michelsonii Hauman. Conclusive evidence for a monofluoroacetate presence came from its isolation from the freeze-dried extract of stem bark. Three free unusual amino acids, named N-methyl-α-alanine, N-methyl-β-alanine, and 2,7-diaminooctan-1,8-dioic acid, described for the first time in a plant, and known trigonelline were also isolated from the stem bark of D. michelsonii. Structure elucidations were mainly achieved by spectroscopic methods (1H-NMR, 2D-NMR, MS) and by comparison with authentic references. These unusual amino acids were detected by a fast, reliable TLC analysis in all our batches of Umutambasha, suggesting that they could be used for identification purposes in case of human or livestock intoxications. Finally, EEG recordings and behavioural observations performed in mice suggested that the convulsive patterns produced by Umutambasha are the consequence of monofluoroacetate presence in D. michelsonii.
Centre Interfacultaire de Recherche du Médicament CIRM ; Center for Cellular and Molecular Neurobiology CNCM
Fonds Léon Fredericq
http://hdl.handle.net/2268/159005
10.1055/s-0032-1328283

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