Article (Scientific journals)
Broad antibiotic resistance profile of the subclass B3 metallo-β-lactamase GOB-1, a di-zinc enzyme.
Horsfall, Louise; Izougarhane, Youssef; Lassaux, Patricia et al.
2011In FEBS Journal, 278(8), p. 1252-1263
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Abstract :
[en] The metallo-β-lactamase (MBL) GOB-1 was expressed via a T7 expression system in Escherichia coli BL21(DE3). The MBL was purified to homogeneity and shown to exhibit a broad substrate profile, hydrolyzing all the tested β-lactam compounds efficiently. The GOB enzymes are unique among MBLs due to the presence of a glutamine residue at position 116, a zinc-binding residue in all known class B1 and B3 MBL structures. Here we produced and studied the Q116A, Q116N and Q116H mutants. The substrate profiles were similar for each mutant, but with significantly reduced activity compared with that of the wild-type. In contrast to the Q116H enzyme, which bound two zinc ions just like the wild-type, only one zinc ion is present in Q116A and Q116N. These results suggest that the Q116 residue plays a role in the binding of the zinc ion in the QHH site.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Horsfall, Louise;  Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Izougarhane, Youssef
Lassaux, Patricia ;  Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Selevsek, Nathalie
Liénard, Benoit
Poirel, Laurent
Kupper, Michaël;  Rheinisch - Westfälische Technische Hochschule Aachen - RWTH
Hoffmann, Kurt;  Rheinisch - Westfälische Technische Hochschule Aachen - RWTH
Frère, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Bebrone, Carine ;  Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Language :
English
Title :
Broad antibiotic resistance profile of the subclass B3 metallo-β-lactamase GOB-1, a di-zinc enzyme.
Publication date :
April 2011
Journal title :
FEBS Journal
ISSN :
1742-464X
eISSN :
1742-4658
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
278(8)
Pages :
1252-1263
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Gouvernement Fédéral Belge [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
European Research Training Network
EU - European Commission
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