p27(Kip1) Is a Microtubule-Associated Protein that Promotes Microtubule Polymerization during Neuron Migration.

[en] The migration of cortical interneurons is characterized by extensive morphological changes that result from successive cycles of nucleokinesis and neurite branching. Their molecular bases remain elusive, and the present work describes how p27(Kip1) controls cell-cycle-unrelated signaling pathways to regulate these morphological remodelings. Live imaging reveals that interneurons lacking p27(Kip1) show delayed tangential migration resulting from defects in both nucleokinesis and dynamic branching of the leading process. At the molecular level, p27(Kip1) is a microtubule-associated protein that promotes polymerization of microtubules in extending neurites, thereby contributing to tangential migration. Furthermore, we show that p27(Kip1) controls actomyosin contractions that drive both forward translocation of the nucleus and growth cone splitting. Thus, p27(Kip1) cell-autonomously controls nucleokinesis and neurite branching by regulating both actin and microtubule cytoskeletons.

Disciplines :

Neurology

Author, co-author :

Godin, Juliette ^✱; Université de Liège - ULiège > Département des sciences cliniques > Neurologie

Thomas, Noemie ^✱

Laguesse, Sophie ^✱; Université de Liège - ULiège > Département des sciences cliniques > Neurologie

Malinouskaya, Lina

Close, Pierre ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

MALAISE, Olivier

Purnelle, Audrey

Raineteau, Olivier

Campbell, Kenneth

Fero, Matthew

More authors (6 more)

^✱ These authors have contributed equally to this work.

Language :

English

Title :

p27(Kip1) Is a Microtubule-Associated Protein that Promotes Microtubule Polymerization during Neuron Migration.

Publication date :

2012

Journal title :

Developmental Cell

ISSN :

1534-5807

Publisher :

Cell Press, United States

Volume :

Issue :

Pages :

729-44

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 26 November 2012

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