Cellobiose; Cellulase binding; Microcrystalline cellulose; Multistage process; Paper pulp; Water retention value
Abstract :
[en] The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.
Research center :
Chimie Biologique Industrielle
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Vanderghem, Caroline ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Jacquet, Nicolas ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Danthine, Sabine ; Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.
Blecker, Christophe ; Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.
Paquot, Michel ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Language :
English
Title :
Effect of physicochemical characteristics of cellulosic substrates on enzymatic hydrolysis by means of a multi-stage process for cellobiose production
Publication date :
March 2012
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press, Totowa, United States - New Jersey
Volume :
166
Issue :
6
Pages :
1423-1432
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
TECHNOSE
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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