[en] We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection of steroid agonists and antagonists down to 0.3 ng g(-1) is described and its application to the analysis of bovine samples is discussed. In particular, the use of the assays to detect illegal progesterone or androgen treatment has to take into account the presence of natural endogenous hormones. (C) 2002 Elsevier Science B.V. All rights reserved.
Disciplines :
Chemistry
Author, co-author :
Willemsen, Philippe
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Maghuin-Rogister, Guy ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Language :
English
Title :
Use of specific bioluminescent cell lines for the detection of steroid hormone (ant)agonists in meat producing animals
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