Molecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61

acyltransferases analysis isolation & purification; amino acid sequence; carboxypeptidases analysis isolation & purification; electrophoresis polyacrylamide gel; isoelectric focusing; molecular weight; peptidoglycan; proteins analysis; streptomyces enzymology

Abstract :

[en] A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R61 to protein homogeneity (95% purity) is described. The isolated protein has a molecular weight of about 38000 and consists of one polypeptide chain. Its amino acid composition is presented.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Frère, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Faculté de Medecine, Institut de Botanique > Service de Microbiologie

Perkins, Harnold R.; National Institute for Medical Research

Nieto, Manuel; National Institute for Medical Research

Language :

English

Title :

Molecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61

Publication date :

01 November 1973

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, London, United Kingdom

Volume :

135

Issue :

Pages :

463-468

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
FRFC - Fonds de la Recherche Fondamentale Collective

Available on ORBi :

since 03 February 2011

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