Article (Scientific journals)
Sensitivity to ampicillin and cephalothin of enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44
Nguyen-Distèche, Martine; Pollock, Jerry J.; Ghuysen, Jean-Marie et al.
1974In European Journal of Biochemistry, 41 (3), p. 457-463
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Keywords :
acyltransferases metabolism; ampicillin pharmacology; bacterial proteins biosynthesis; carboxypeptidases metabolism; cell membrane enzymology; cell wall/metabolism; cephalothin pharmacology; dose-response relationship, drug; escherichia coli/cytology/drug effects enzymology; tethal dose 50; microscopy, phase-contrast; mutation; nitrosoguanidines/pharmacology; penicillin g/pharmacology; penicillinase/metabolism; peptide hydrolases/*metabolism; rifampin/pharmacology; surface-active agents
Abstract :
[en] After extraction of the membranes of Escherichia coli K12 strain 44 by Brij-36T, each of the four enzyme activities (natural transpeptidase, unnatural transpeptidase, carboxypeptidase and endopeptidase) of the wall peptide crosslinking system, occurs in two forms characterized by large differences in their sensitivity to ampicillin (but much smaller differences in their sensitivity to cephalothin). The fractionation of the enzyme activities into two groups of low and high sensitivity to ampicillin is achieved essentially by chromatography of the membrane extract on DEAE-cellulose.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Pollock, Jerry J.;  New York University School of Medicine > Department of Microbiology
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Puig, Juan;  Universitad de Los Andes > Faculdad de Ciencia > Departamento de Biologia
Reynolds, Peter;  University of Cambridge > Department of Biochemistry > Sub-Department of Chemical Microbiology
Perkins, Harnold-R.;  University of Cambridge > Department of Biochemistry > Sub-Department of Chemical Microbiology
Coyette, Jean;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Salton, Milton-R-J.;  National Institute for Medical Research
Language :
English
Title :
Sensitivity to ampicillin and cephalothin of enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44
Publication date :
01 February 1974
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
41
Issue :
3
Pages :
457-463
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
Available on ORBi :
since 28 January 2011

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