Article (Scientific journals)
The repressing function of the oncoprotein BCL-3 requires CtBP while its polyubiquitination and degradation involve the E3 ligase TBLR1
Keutgens, Aurore; Shostak, Kateryna; Close, Pierre et al.
2010In Molecular and Cellular Biology, 30, p. 4006-4021
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Keywords :
NF-kappa B; BCL-3; polyubiquitination
Abstract :
[en] The nuclear and oncogenic BCL-3 protein activates or represses gene transcription when bound to NF-kB proteins p50 and p52, yet the molecules that specifically interact with BCL-3 and drive BCL-3-mediated effects on gene expression remain largely uncharacterized. Moreover, GSK3-mediated phosphorylation of BCL-3 triggers its degradation through the proteasome, but the proteins involved in this degradative pathway are poorly characterized. Biochemical purification of interacting partners of BCL-3 led to the identification of CtBP as a molecule required for the ability of BCL-3 to repress gene transcription. CtBP is also required for the oncogenic potential of BCL-3 and for its ability to inhibit UV-mediated cell apoptosis in keratinocytes. We also defined the E3 ligase TBLR1 as a protein involved in BCL-3 degradation through a GSK3-independent pathway. Thus, our data demonstrate that the LSD1/CtBP complex is required for the repressing abilities of an oncogenic IkB protein, and they establish a functional link between the E3 ligase TBLR1 and NF-kB.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Keutgens, Aurore ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Shostak, Kateryna ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Close, Pierre  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Zhang-Shao, Xin ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Hennuy, Benoît ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transcriptomique
Aussems, Marie
Chapelle, Jean-Paul ;  Université de Liège - ULiège > Chimie médicale
Viatour, Patrick ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Gothot, André ;  Université de Liège - ULiège > Hématologie biologique et immuno hématologie
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Chariot, Alain ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Language :
English
Title :
The repressing function of the oncoprotein BCL-3 requires CtBP while its polyubiquitination and degradation involve the E3 ligase TBLR1
Publication date :
August 2010
Journal title :
Molecular and Cellular Biology
ISSN :
0270-7306
eISSN :
1098-5549
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
30
Pages :
4006-4021
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS, TELEVIE, Fédération Belge contre le Cancer
Available on ORBi :
since 05 September 2010

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