Article (Scientific journals)
Binding Site-Shaped Repeated Sequences of Bacterial Wall Peptidoglycan Hydrolases
Ghuysen, Jean-Marie; Lamotte-Brasseur, Josette; Joris, Bernard et al.
1994In FEBS Letters, 342 (1), p. 23-28
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Abstract :
[en] The non-catalytic C-terminal regions of the N-acetylmuramidase (lysozyme) of Clostridium acetobutylicum and N-acetylmuramoyl(D-lactyl)-L-alanine amidases CwlA of Bacillus subtilis, ORFL3 and CwlL of Bacillus licheniformis were previously reported to have similarities with the amino acid sequence of the non-catalytic N-terminal module of the Streptomyces albus G Zn DD-peptidase. This peptidase is a bipartite protein of known three-dimensional structure. Its non-catalytic N-terminal module possesses, exposed at the surface, an elongated crevice which is defined by a loop-helix-loop-helix motif that consists of two repeats, each 16 amino acid residues long, connected by a heptapeptide and whose design is compatible with its possible functioning as a substrate recognition and binding site. Amino acid alignments suggest that cavities nearly identical in shape to that present in the non-catalytic module of the S. albus peptidase, are borne by the C-terminal regions of the CwlA amidase (in one copy), the lysozyme and the ORFL3 and CwlL amidases (in two copies). Since a common feature of the five enzymes is their substrate, the bacterial cell wall peptidoglycan, we interpret the striking similarity of their non-catalytic N- or C-terminal modules to suggest that these modules are involved in the binding of these exocellular enzymes to their insoluble wall substrate.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Institut de Chimie > Centre d'ingénieurie des Protéines
Lamotte-Brasseur, Josette ;  Université de Liège - ULiège > Institut de Chimie > Centre d'ingénierie des Protéines
Joris, Bernard ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Shockman, Gérald D.;  Temps University School of Medecine-Philadelphia > Department of Microbiology and Immunology
Language :
English
Title :
Binding Site-Shaped Repeated Sequences of Bacterial Wall Peptidoglycan Hydrolases
Publication date :
28 March 1994
Journal title :
FEBS Letters
ISSN :
0014-5793
eISSN :
1873-3468
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
342
Issue :
1
Pages :
23-28
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 September 2010

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