Reference : Cloning, Nucleotide Sequence and Amplified Expression of the Gene Encoding the Extrac...
Scientific journals : Article
Life sciences : Microbiology
Cloning, Nucleotide Sequence and Amplified Expression of the Gene Encoding the Extracellular Metallo (Zn) Dd-Peptidase of Streptomyces Albus G
Duez, Colette mailto [Université de Liège - ULiège > > Centre d'ingénierie des protéines >]
Lakaye, Bernard mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
Houba, Simone [> > > >]
Dusart, Jean [> > > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Service de microbiologie]
FEMS Microbiology Letters
Yes (verified by ORBi)
[en] Zn DD-peptidase ; Peptidoglycan ; streptomyces albus G
[en] The gene encoding the extracellular metallo (Zn) DD-peptidase of Streptomyces albus G has been cloned in Escherichia coli DH5 alpha MCR via pBR322 or 325, and then transferred into Streptomyces lividans TK24 via pIJ486, with substantial amplification of the expressed DD-peptidase. The gene has the information for the synthesis of a 255 amino acid precursor, the amino terminal region of which has the characteristic features of a signal peptide. The primary structure as deduced from nucleotide sequencing confirms that previously determined by chemical methods except for the occurrence of an Asp instead of Asn at position 1 and an additional Ala immediately downstream of Pro67.
Centre d'ingénierie des protéines - CIP
Researchers ; Professionals

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