Article (Scientific journals)
Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular β-lactamase of Actinomadura R39
Fraipont, Claudine; Duez, Colette; Matagne, André et al.
1989In Biochemical Journal, 262 (3), p. 849-854
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Keywords :
clonage, surexpression; beta-lactamase; Streptomyces lividans
Abstract :
[en] By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone in S. lividans TK24, the gene that encodes the Actinomadura R39, β-lactamase. Gene cloning resulted in an amplified expression of the , β lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg . litre of culture-1).
Disciplines :
Microbiology
Author, co-author :
Fraipont, Claudine ;  Université de Liège - ULiège > Département de Microbiologie
Duez, Colette ;  Université de Liège - ULiège > Département de Microbiologie
Matagne, André  ;  Université de Liège - ULiège > Département de Microbiologie
Molitor, Chantal;  Université de Liège - ULiège > Département de Microbiologie
Dusart, Jean;  Université de Liège - ULiège > Département de Microbiologie
Frère, Jean-Marie ;  Université de Liège - ULiège > Département de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Département de Microbiologie
Language :
English
Title :
Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular β-lactamase of Actinomadura R39
Publication date :
1989
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
262
Issue :
3
Pages :
849-854
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 27 December 2010

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