[en] By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone in S. lividans TK24, the gene that encodes the Actinomadura R39, β-lactamase. Gene cloning resulted in an amplified expression of the , β lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg . litre of culture-1).
Disciplines :
Microbiology
Author, co-author :
Fraipont, Claudine ; Université de Liège - ULiège > Département de Microbiologie
Duez, Colette ; Université de Liège - ULiège > Département de Microbiologie
Matagne, André ; Université de Liège - ULiège > Département de Microbiologie
Molitor, Chantal; Université de Liège - ULiège > Département de Microbiologie
Dusart, Jean; Université de Liège - ULiège > Département de Microbiologie
Frère, Jean-Marie ; Université de Liège - ULiège > Département de Microbiologie
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Département de Microbiologie
Language :
English
Title :
Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular β-lactamase of Actinomadura R39
Publication date :
1989
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
262
Issue :
3
Pages :
849-854
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]