Automated determination of pirlindole enantiomers in plasma by on-line coupling of a pre-column packed with restricted access material to a chiral liquid chromatographic column
[en] A fully automated liquid chromatographic method has been developed for the determination of the enantiomers of pirlindole, an antidepressant drug, in human plasma. The method is based on the use of a pre-column packed with restricted access material (RAM) (LiChrospher ADS RP-4) for sample clean-up coupled to a column containing a cellulose tris-(3,5-dimethylphenylcarbamate) based chiral stationary phase (Chiralcel OD-R) for the separation and quantitative analysis of pirlindole enantiomers. A 50-microl plasma volume was injected directly onto the pre-column using a mixture of phosphate buffer (pH 5.0) and methanol (97:3; v/v) as washing liquid. By rotation of a switching valve, the analytes were then eluted in the back-flush mode with the LC mobile phase. A complete separation of pirlindole enantiomers was obtained in 22 min on the Chiralcel OD-R column, using a mobile phase made of a mixture of phosphate buffer (pH 5.0) containing 50 mM sodium perchlorate and acetonitrile (65:35; v/v). The flow-rate was 0.6 ml/min and the analytes were detected fluorometrically using 295 and 340 nm as excitation and emission wavelengths, respectively. The method was then validated and was found to be linear in the 2.5-200 ng/ml range. The limit of detection was lower than 1 ng/ml. Repeatability and intermediate precision at a concentration of 50 ng/ml were about 1.5 and 3.5%, respectively.
Disciplines :
Chemistry Pharmacy, pharmacology & toxicology
Author, co-author :
Chiap, Patrice ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Ceccato, Attilio ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Automated determination of pirlindole enantiomers in plasma by on-line coupling of a pre-column packed with restricted access material to a chiral liquid chromatographic column
Publication date :
15 January 2002
Journal title :
Journal of Pharmaceutical and Biomedical Analysis
ISSN :
0731-7085
eISSN :
1873-264X
Publisher :
Pergamon-Elsevier Science Ltd, Oxford, United Kingdom
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Medvedev A., Gorkin V., Shvedov V., Fedotova O., Semiokhina A. Drug Invest. 1992, 4:501-507.
Bruhwyler J., Liégeois J.-F., Géczy J. Pharmacol. Res. 1993, 6:23-33.
De Wilde J., Geerts S., Van Dorpe J., Bruhwyler J., Géczy J. Acta Psychiatr. Scand. 1996, 94:404-410.
Vielhauer S., Rudolphi A., Boos K.-S., Seidel D. J. Chromatogr. B 1995, 666:315-322.
Rudolphi A., Vielhauer S., Boos K.-S., Seidel D., Bäthge I.M., Berger H. J. Pharm. Biomed. Anal. 1995, 13:615-623.
Yu Z., Westerlund D. J. Chromatogr. A 1996, 725:149-155.
Van der Hoeven R.A.M., Hofte A.J.P., Frenay M., Irth H., Tjaden U.R., Van der Greef J., Rudolphi A., Boos K.-S., Marko Varga G., Edholm L.E. J. Chromatogr. A 1997, 762:193-200.
Oertel R., Richter K., Gramatté T., Kirch W. J. Chromaogr. A 1998, 797:203-209.
Ceccato A., Boulanger B., Chiap P., Hubert Ph., Crommen J. J. Chromatogr. A 1998, 819:143-153.
Ceccato A., Toussaint B., Chiap P., Hubert Ph., Crommen J. Enantiomer 1999, 4:305-315.
Gordi T., Nielsen E., Yu Z., Westerlund D., Ashton M. J. Chromatogr. B 2000, 742:155-162.
Baeyens W.R.G., Van der Weken G., Haustraete J., Aboul-Enein H.Y., Corveleyn S., Remon J.P., García-Campaña A.M., Deprez P. J. Chromatogr. A 2000, 871:153-161.
Öhman D., Carlsson B., Norlander B. J. Chromatogr. B 2001, 753:365-373.
Ostrowski J., Theumer J., Gärtner W., Resag K. J. Chromatogr. 1984, 309:115-123.
Ceccato A., Hubert Ph., De Tullio P., Liégeois J.-F., Stachow M., Géczy J., Crommen J. J. Pharm. Biomed. Anal. 1998, 17:1071-1079.
Ceccato A., Hubert Ph., De Tullio P., Liégeois J.-F., Felikidis A., Géczy J., Crommen J. J. Pharm. Biomed. Anal. 1998, 18:605-614.
De Tullio P., Felikidis A., Pirotte B., Liégeois J.-F., Stachow M., Delarge J., Ceccato A., Hubert Ph., Crommen J., Géczy J. Helv. Chim. Acta 1998, 81:539-547.
LiChrospher® ADS Application Guide, Merck; 2000.
Shah V.P., Midha K.K., Dighe S., McGilveray I.J., Skelly J.P., Yacobi A., Layloff T., Viswanathan C.T., Cook C.E., McDowall R.D., Pittman K.A., Spector S. Pharm. Res. 1992, 9:588-592.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.