Reference : Intrasaccular injection of aminoglycosides: a novel method for temporary damaging fis...
Scientific congresses and symposiums : Poster
Life sciences : Environmental sciences & ecology
Intrasaccular injection of aminoglycosides: a novel method for temporary damaging fish inner ear hair cells
Faucher, Karine mailto [Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Morphologie fonctionnelle et évolutive >]
Aas-Hansen, Øyvind [> > >]
Damsgard, Borge [> > >]
Stenklev, Niels-Christian [> > >]
Benelux Congress of Zoology
du 30 octobre 2008 au 31 octobre 2008
Université de Liège
[en] Fish models are increasingly being used for hearing research investigations. Aminoglycoside antibiotics that are used for damaging the inner ear hair cells can have systemic side effects leading to death of study animals. This study aimed to compare two methods: i) systemic (intravenous) and ii) local (intrasaccular) gentamicin administration for induction of inner ear hair cell damage in the Atlantic cod, Gadus morhua (L.). Hair cell damage was assessed using scanning electron microscopy; hair cell density, prevalence of immature hair cells and kinocilia length were measured. Gentamicin-treated fish were compared with control and sham fish. Intravenous gentamicin led to dose-dependent mortality caused by nephrotoxicity. The only visible effect after treatment was more immature hair cells and shorter kinocilia, the effect on hair cell density was equivocal. Following intrasaccular gentamicin treatment, fish mortality was negligible, and hair cells were damaged regardless of dose. Here, we observed decreased hair cell density, high prevalence of immature hair cells, and significantly shortened kinocilia. Conclusion: intrasaccular injection is preferable to intravenous injection of gentamicin for the study of ototoxicity in the Atlantic cod.
Laboratoire de Morphologie Fonctionnelle et Evolutive
Researchers ; Professionals ; Students

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