Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells.

Cornez, Isabelle; Creppe, Catherine; Gillard, Magali; Hennuy, Benoît; Chapelle, Jean-Paul; Dejardin, Emmanuel; Merville, Marie-Paule; Close, Pierre; Chariot, Alain

doi:10.1016/j.bcp.2008.03.006

Article (Scientific journals)

Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells.

Cornez, Isabelle; Creppe, Catherine; Gillard, Magali et al.

2008 • In Biochemical Pharmacology, 75, p. 2122-2134

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/3687

DOI
10.1016/j.bcp.2008.03.006

PubMed
18430410

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Keywords :

Elongator; p53; transcription

Abstract :

[en] Elongator, a multi-subunit complex assembled by the IkappaB kinase-associated protein (IKAP)/hELP1 scaffold protein is involved in transcriptional elongation in the nucleus as well as in tRNA modifications in the cytoplasm. However, the biological processes regulated by Elongator in human cells only start to be elucidated. Here we demonstrate that IKAP/hELP1 depleted colon cancer-derived cells show enhanced basal expression of some but not all pro-apoptotic p53-dependent genes such as BAX. Moreover, Elongator deficiency causes increased basal and daunomycin-induced expression of the pro-survival serum- and glucocorticoid-induced protein kinase (SGK) gene through a p53-dependent pathway. Thus, our data collectively demonstrate that Elongator deficiency triggers the activation of p53-dependent genes harbouring opposite functions with respect to apoptosis.

Research Center/Unit :

Giga-Signal Transduction - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Cornez, Isabelle ; Université de Liège - ULiège > Chimie médicale

Creppe, Catherine ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Gillard, Magali ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Hennuy, Benoît ; Université de Liège - ULiège > GIGA-Management : Plate-forme transcriptomique

Chapelle, Jean-Paul ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Dejardin, Emmanuel ; Université de Liège - ULiège > Virologie - Immunologie

Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Close, Pierre ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Chariot, Alain ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Language :

English

Title :

Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells.

Publication date :

June 2008

Journal title :

Biochemical Pharmacology

ISSN :

0006-2952

eISSN :

1873-2968

Publisher :

Elsevier Science, Oxford, United Kingdom

Volume :

Pages :

2122-2134

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
Télévie
FBC
ULg. ARC - Université de Liège. Actions de Recherche Concertées
BELSPO - Belgian Science Policy Office

Funding text :

PAI

Available on ORBi :

since 06 January 2009

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Bibliography

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