Reference : Influence of culture conditions on lipopeptide production by Bacillus subtilis
Scientific journals : Article
Life sciences : Agriculture & agronomy
http://hdl.handle.net/2268/36361
Influence of culture conditions on lipopeptide production by Bacillus subtilis
English
Akpa, E. [> > > >]
Jacques, Philippe mailto [> > > >]
Wathelet, Bernard mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Paquot, Michel mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Fuchs, R. [> > > >]
Budzikiewicz, H. [> > > >]
Thonart, Philippe mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
2001
Applied Biochemistry and Biotechnology
Humana Press
91-3
551-561
Yes (verified by ORBi)
International
0273-2289
Totowa
NJ
[en] Bacillus subtilis produces various families of lipopeptides with different homologous compounds. To produce "new molecules" with improved activities and to select strains that produced a reduced number of homologs or isomers, we studied the effects of different media on the nature of the synthesis of fatty; acid chains for each lipopeptide family. This study focused on two B. subtilis strains cultivated in flasks. Optimized medium for lipopeptide production and Landy medium modified by replacing glutamic acid with other alpha -amino acids were used. We found that the intensity of production of homologous compounds depends on the strain and the culture medium. Analysis of these lipopeptides by high-performance liquid chromatography showed that the strain R. subtilis NT02 yielded various homologous compounds when cultivated in Landy medium (L-Glu), but primarily one homologous product in hi,oh relative amounts when cultivated in the optimized medium. Mass spectrometric analysis and determination of the amino acid composition of this molecule enabled us to identify it as Bacillomycine L c15.
http://hdl.handle.net/2268/36361
10.1385/ABAB:91-93:1-9:551

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