Optimization of a miniaturized microwave-assisted saponification and extraction method to evaluate mosh and MOAH in biological samples

Albendea, Paula; Gorska, Aleksandra; Purcaro, Giorgia

doi:10.1016/j.jcoa.2025.100267

Article (Scientific journals)

Optimization of a miniaturized microwave-assisted saponification and extraction method to evaluate mosh and MOAH in biological samples

Albendea, Paula; Gorska, Aleksandra; Purcaro, Giorgia

2025 • In Journal of chromatography open, 8, p. 100267

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/348423

DOI
10.1016/j.jcoa.2025.100267

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Keywords :

Biological samples; LC-GC-FID; LC-GC×GC-FID/MS; Mineral oil aromatic hydrocarbons; Mineral oil saturated hydrocarbons; Miniaturized saponification; Analytical Chemistry

Abstract :

[en] Mineral oil hydrocarbons (MOH) are a group of lipophilic contaminants composed of thousands of different compounds. The human toxicokinetics (absorption, metabolization, distribution and elimination) of these contaminants depends on their molecular structure but information are still lacking. The primary gap to be filled is the optimization and validation of a method to treat a very small amount of samples, as the ones produced during in-vitro studies. The aim of this study is to develop and validate a miniaturized microwave-assisted saponification and extraction (MASE) method for the evaluation of MOH subclasses in low amounts of biological samples (<400 mg). The method was validated using an LC/GC×GC-FID/MS system in mono- and two-dimensional modes. The results demonstrated excellent recovery (≥94 %), repeatability, and compliance with JRC (Joint Research Center) guidance criteria for internal standard ratios. Furthermore, the method achieved a limit of quantification as low as 0.4 µg of MOH in the sample, outperforming conventional extraction methods.

Disciplines :

Chemistry
Food science

Author, co-author :

Albendea, Paula ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)

Gorska, Aleksandra ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)

Purcaro, Giorgia ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)

Language :

English

Title :

Optimization of a miniaturized microwave-assisted saponification and extraction method to evaluate mosh and MOAH in biological samples

Publication date :

November 2025

Journal title :

Journal of chromatography open

eISSN :

2772-3917

Publisher :

Elsevier B.V.

Volume :

Pages :

100267

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S2772391725000659?httpAccept=text/xml

Funders :

F.R.S.-FNRS - Fund for Scientific Research

Funding text :

The authors thank LECO, Restek, and Milestone for their support. This article is based upon work from the Sample Preparation Study Group and Network, supported by the Division of Analytical Chemistry of the European Chemical Society. This work is supported by ACESSS (Academic Center of Excellence for Separation Science and Sensing).This work was supported by Fonds de la Recherche Scientifique Belgique (FNRS) (PDR projects-ToxAnaMOH T.0187.23).

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