[en] The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots.
Dunlop D and Curtis W (1991) Synergistic response of plant hairy-root cultures to phosphate limitation and fungal elicitation. Biotechnol Prog 7: 434-438
Flores H and Medina-Bolivar F (1995) Root culture and plant natural products: "Unearthing" the hidden half of plant metabolism. Plant Tissue Cult and Biotechnol 1: 59-74
Furuya T and Ushiyama K (1994) Ginseng production in cultures of Panax ginseng cells. In: Shargool PD and Ngo TT (eds) Biotechnological Application of Plant Cultures. USA: CRC Press, pp 1-22
Gaspar Th (1995) Seleniated forms of indolylacetic acid. New powerful synthetic auxins. Acros Organics Acta 1: 65-66
Gaspar Th, Kevers C, Penel C, Greppin H, Reid D and Thorpe TA (1996) Plant hormones and plant growth regulators in plant tissue cultures. In Vitro Cell Dev Biol Plant 32: 272-289
Hausman JF, Kevers C and Gaspar Th (1994) Involvement of putrescine in the inductive rooting phase of poplar shoots raised in vitro. Physiol Plant 92: 201-206
Hofinger M, Thorpe Th, Bouchet M and Gaspar Th (1980) Auxin-like activity of (benzo(b) selenienyl-3) acetic acid. Acta Physiol Plant 2: 275-280
Hu SY (1976) The genus Panax (ginseng) in Chinese medicine. Econ Bot 30: 11-28
Hwang B and Ko KM (1989) Induction and culture of hairy roots from ginseng (Panax ginseng C.A. Meyer) roots discs by A. rhizogenes. Kor J Biotechnol Bioeng 4: 288-292
Hwang B, Ko KM, Hwang KH, Hwang SJ and Kang H (1991) Production of saponin by hairy root cultures of ginseng (Panax ginseng C.A. Meyer) transformed with Agrobacterium rhizogenes. Kor J Bot 34: 289-296
Hwang B, Ko KM, Yang DC, Park JC and Choi KT (1997) The enhancing method of ginsenoside production for hairy root mass cultures of ginseng (Panax ginseng C.A. Meyer). In: Altman A and Waisel Y (eds) Biology of Root Formation and Development. New York: Plenum Press, p. 325
Kevers C, Bringaud C, Hausman JF and Gaspar Th (1997) Putrescine involvement in the inductive phase of walnut shoots rooting in vitro. Saussurea 28: 47-57
Kevers C, Hausman JF, Faivre-Rampant O, Evers D and Gaspar Th (1997) Hormonal control of adventitious rooting: Progress and questions. J Appl Bot/Angew Bot 71: 71-79
Ko KM, Song JJ, Hwang B and Kang YH (1993) Cytogenetic and histological characteristics of ginseng hairy root transformed by Agrobacterium rhizogenes. Kor J Bot 36: 75-81
Ko KM, Ahn JC, Hwang SJ, Kang YH and Hwang B (1993) Production of secondary metabolites from hairy root of Panax ginseng transformed by Agrobacterium rhizogenes. II. Improvement of saponin contents and mass cultures of ginseng hairy root. Kor J Plant Tissue Cult 20: 41-46
Ko KM, Choi YS and Hwang B (1994) Production and identification of anthocyanin in hairy root cultures of ginseng. J Plant Biol 37: 85-91
Ko KM, Yang DC, Park JC, Choi KJ, Choi KT and Hwang B (1996) Mass culture and ginsenoside production of ginseng hairy root by two-step culture process. J Plant Biol 39: 63-69
Lamproye A, Hofinger M, Berthon JY and Gaspar Th (1990) [Benzo(b)selenienyl-3] acetic acid: A potent synthetic auxin in somatic embryogenesis. Comptes Rendus Acad Sc Paris Sér III 311: 127-132
Lee JS, Ko KM, Ahn JC, Bai DG, Park KY, Ko R and Hwang B (1994) High yield saponin production by mass cultures of ginseng tranformed tissue. I. Induction, culture of transformed tissue and selection of high-saponin-producing clones in ginseng. Kor J Biotechnol Bioeng 9: 157-164
Lee HS, Kim SW, Li KW, Eriksson T and Liu JR (1995) Agrobacterium-mediated transformation of ginseng (Panax ginseng) and mitotic stability of the inserted β-glucuronidase gene in regenerants from isolated protoplasts. Plant Cell Rep 14: 545-549
Li TSC (1995) Asian and American ginseng: A review. HortTechnology 5: 27-34
Mathur A, Shukla N, Pal M, Ahuja PS and Uniyal GC (1994) Saponin production in callus and cell suspension cultures of Panax quinquefolium. Phytochem 34: 1221-1225
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497
Ripetti V, Kevers C and Gaspar Th (1994) Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and in ethylene production. Advances Hortic Science 8: 29-32
Signs M and Flores H (1990) The biosynthetic potential of plant roots. BioAssays 12: 7-13
Yoshikawa T and Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep 6: 449-453
Yoshimatsu K, Yamaguchi H and Shimomura K (1996) Traits of Panax ginseng hairy roots after cold storage and cryopreservation. Plant Cell Rep 15: 555-560
