Flow cytometry estimation of nuclear size and ploidy level of habituated calli of sugar beet

Kevers, Claire; Greimers, Roland; Franck, Thierry; Bisbis, Badia; Dommes, Jacques; Gaspar, Thomas

doi:10.1023/A:1002469331895

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Flow cytometry estimation of nuclear size and ploidy level of habituated calli of sugar beet

Kevers, Claire; Greimers, Roland; Franck, Thierry et al.

1999 • In Biologia Plantarum, 42 (3), p. 321-332

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10.1023/A:1002469331895

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Keywords :

Beta vulgaris; habituation; aneuploidy

Abstract :

[en] A fully habituated (auxin- and cytokinin-independent) self-regenerating (organogenic) sugar beet cell line (HO) and a fully habituated non-organogenic one (HNO) derived from the former one, were analyzed as to their nuclear size and DNA content. Flow cytometry and image analysis were used and cells of certified diploid leaves of the same sugar beet strain served as controls. The HNO cells had been shown previously to have many characteristics of cancerous cells. The analyses made on leaves and HNO cells indicated the presence of only one population of cycling cells. In HO cells. two cycling populations were detected: the first one had the same DNA content as the leaves while the second one contained two fold more DNA than the first population. HNO cells showed the higher nuclear size and DNA content. HNO cells also showed evidence of aneuploidy. Thus, nuclear size, DNA content and ploidy level increase together with the neoplasic progression to culminate in HNO cells with the loss of organogenic totipotency.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Kevers, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales

Greimers, Roland ; Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique

Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Bisbis, Badia; Université de Liège - ULiège > Hormonologie végétale

Dommes, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales

Gaspar, Thomas ; Botanique, radiobotanique & serres expér.

Language :

English

Title :

Flow cytometry estimation of nuclear size and ploidy level of habituated calli of sugar beet

Publication date :

1999

Journal title :

Biologia Plantarum

ISSN :

0006-3134

eISSN :

1573-8264

Publisher :

Kluwer Academic Publishers, Dordrecht, Netherlands

Volume :

Issue :

Pages :

321-332

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 03 January 2009

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