A comparison of respiratory pathways in fully habituated and normal non-organogenic sugarbeet callus

[en] The respiration of cells and isolated mitochondria of a habituated non-organogenic (HNO) cell line (auxin- and cytokinin-independent) and of a normal (N) cell line (auxin- and cytokinin-requiring) from sugarbeet (Beta vulgaris L.) was investigated. Oxygen uptake by both cells and mitochondria of the HNO fine was higher than that of the N line. Respiration in the presence of cyanide (i.e. capacity of the alternative pathway) was also higher in the HNO callus as compared with the N one. The measurements of O-2 uptake from isolated HNO and N mitochondria showed that addition of a mixture of substrates (NADH, succinate, malate and NAD) resulted in a higher respiration via the CN-resistant pathway in mitochondria From HNO cells. The activity of cytochrome c oxidase was twice as high as in the N cells as compared with the HNO cells. Immunoblots also showed that a higher alternative oxidase protein was present in HNO cells than in the N cells. A model showing the relationships between the different metabolic pathways previously studied in the HNO cells and the higher respiration via the CN-resistant pathway in the HNO cells is proposed.

Disciplines :

Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Bisbis, Badia; Université de Liège - ULiège > Hormonologie Végétale

Wagner, Anneke; Vrije Universiteit Amsterdam > Molecular cell physiology

Kevers, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales

Gaspar, Thomas ; Botanique, radiobotanique & serres expér.

Language :

English

Title :

A comparison of respiratory pathways in fully habituated and normal non-organogenic sugarbeet callus

Publication date :

2000

Journal title :

Journal of Plant Physiology

ISSN :

0176-1617

Publisher :

Urban & Fischer, Jena, Germany

Volume :

156

Issue :

Pages :

312-318

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 January 2009

Statistics

Number of views

35 (1 by ULiège)

Number of downloads

2 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Arron, G. P., and G. E. Edwards: Oxidation of reduced nicotinamide adenine nucleotide phosphate by potato mitochondria. Plant Physiol. 65, 591-594 (1980).
Bisbis, B., F. Le Dily, C. Kevers, J. P. Billard, C. Huault and Th. Gaspar: Disturbed sugar metabolism in a fully habituated non-organogenic callus of Beta vulgaris L. Plant Growth Regul. 11, 257-261 (1993).
Bisbis, B., E. Dujardin, C. Kevers, D. Hagège and Th. Gaspar: Chlorophylls and carotenoids in a fully habituated non-organogenic callus of Beta vulgaris. Biol. Plant. 36, 443-449 (1994).
Bisbis, B., C. Kevers and Th. Gaspar: Atypical TCA cycle and replenishment in a non-photosynthetic fully habituated sugarbeet callus overproducing polyamines. Plant Physiol. Biochem. 35, 363-368 (1997a).
Bisbis, B., C. Kevers, C. Huault, J. P. Billard and Th. Gaspar: Erythromycin as a tool to investigate the tetrapyrrole biosynthetic pathway in habituated and normal sugarbeet calli. Plant Growth Regul. 23, 147-152 (1997b).
Bisbis, B., C. Kevers, C. Penel, H. Greppin and Th. Gaspar: Coexistence of the Beale and the Shemin pathways for the tetrapyrrole-containing compounds' synthesis, including peroxidases, in a normal sugarbeet callus. In: Plant Peroxidase Newsletter, Univ. of Geneva 9, 13-20 (1997c).
Bisbis, B.: Auxin, ethylene and polyamine interactions in a habituated sugarbeet callus. Bull. Soc. Roy. Sc. Liège 67, 213 (1998).
Bisbis, B., C. Kevers, J. Crèche, M. Rideau and Th. Gaspar: Differential growth dependency of normal and habituated sugarbeet cell lines upon endogenous ethylene production and exogenous ethylene application. Physiol. Plant. 103, 201-208 (1998).
Bradford, M. M.: A rapid method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 (1976).
Carrié, B., B. Bisbis, C. Penel, Th. Gaspar and H. Greppin: Disturbed sugar metabolism in a fully habituated non-organogenic callus of Beta vulgaris. Additional data. Saussurea 25, 143-151 (1995).
Carrié, B., Th. Gaspar, H. Greppin and C. Penel: Redox characteristics of normal and habituated cell lines of sugarbeet. Plant Cell Environ. 17, 457-461 (1994).
Crèvecoeur, M., D. Hagège, A. M. Catesson, H. Greppin and Th. Gaspar: Ultrastructural characteristics of cells from normal and habituated sugarbeet calli. Plant Physiol. Biochem. 30, 87-95 (1992).
Gaspar, Th., B. Bisbis, C. Kevers, C. Penel, H. Greppin, F. Le Dily, J.P. Billard, C. Huault, F. Garnier, M. Rideau and J. M. Foidart: Atypical metabolisms and biochemical cycles imposing the cancerous state on plant cells. Plant Growth Regul. 24, 135-144 (1998).
Gaspar, Th., D. Hagège, C. Kevers, C. Penel, M. Crèvecoeur, I. Engelmann, H. Greppin and J. M Foidart: When plant teratomas turn into cancers in the absence of pathogens. Physiol. Plant. 83, 696-670 (1991).
Hagège, D., R. Catania, H. Micalef and Th. Gaspar: Nuclear shape and DNA content of fully habituated non-organogenic sugarbeet cells. Protoplasma 166, 49-54 (1992a).
Hagège, D., D. Werck-Reichhart, P. Schmitt and Th. Gaspar: Deficiency in tetrapyrrole-containing compounds in a non-organogenic habituated sugarbeet cell line. Plant Physiol. Biochem. 30, 649-654 (1992b).
Hagège, D., C. Kevers, Th. Gaspar and T. A. Thorpe: Abnormal growth of habituated sugarbeet callus and cell suspension. In Vitro Cell Dev. Biol. 27, 112-116 (1991).
Hagège, D., C. Kevers, J. Boucaud, M. Duyme and Th. Gaspar: Polyamines, phospholipids and peroxides in normal and habituated sugarbeet calli. J. Plant Physiol. 136, 641-645 (1990).
Kevers, C., B. Bisbis, O. Faivre-Rampant and Th. Gaspar: Putrescine metabolism in a fully habituated nonorganogenic sugarbeet callus and its relationship with growth. J. Plant Physiol. 154, 503-508 (1999).
Lambers, H.: Respiration in intact plants and tissues: its regulation and dependence on environmental factors, metabolism and invaded organisms. In: Douce, R., and D. A. Day (eds.): Encyclopedia of Plant Physiology, New Series, vol. 18, pp. 418-465. Springer Verlag, New York (1985).
Larhrissi, Z., J. L. Bomsel and R. Calvayrac: Relative effect of 2,4-dinitrophenol on the respiratory chain of heterotrophic Euglena gracilis adapted and non-adapted to antimycin. J. Plant Physiol. 143, 264-268 (1994).
Le Dily, F., J. P. Billard, Th. Gaspar and C. Huault: Disturbed nitrogen metabolism associated with the hyperhydric status of fully habituated callus of sugarbeet. Physiol. Plant. 88, 129-134 (1993).
Møller, I. M., and W. Lin: Membrane-bound NAD(P)H dehydrogenases in higher plant cells. Annu. Rev. Plant Physiol. 37, 309-334 (1986).
Moore, A. L., and J. N. Siedow: The regulation and nature of the cyanide-resistant alternative oxidase of plant mitochondria. Biochim. Biophys. Acta 1059, 121-140 (1991).
Poder, D., O. Omarzad and D. Hagège: Comparative study of respiratory metabolism in three types of sugarbeet calli, a normal and two habituated ones (organogenic and non-organogenic). C. R. Acad. Sci. Paris, Sciences de la vie, 321, 479-483 (1998).
Purvis, A. C., and R. L. Shewfelt: Does the alternative pathway ameliorate chilling injury in sensitive plant tissues? Physiol. Plant. 88, 712-718 (1993).
Roberts, T. M., K. M. Fredlund and I. M. Møller: Direct evidence for the presence of two external NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria. FEBS Lett. 373, 307-309 (1995).
Storey, B. T.: Respiratory chain of plant mitochondria. XVIII. Point of interaction of the alternative oxidase with the respiratory chain. Plant Physiol. 58, 521-525 (1976).
Umbach, A. L., and J. N. Siedow: The reaction of soybean cotyledon mitochondrial cyanide-resistant oxidase with sulfhydryl reagent suggests that a-ketoacid activation involves the formation of a thiohemiacetal. J. Biol. Chem. 271, 25019-25026 (1996).
Vanlerberghe, G. C., and L. McIntosh: Coordinate regulation of cytochrome and alternative pathway respiration in tobacco. Plant Physiol. 100, 1846-1851 (1992).
Van Emmerik, W. A. M., A. M. Wagner, J. H. Zwiers and L. H. W. Van Der Plas: The regulation of respiration in cell suspension of Petunia hybrida, studied by inhibiting mitochondrial protein synthesis with chloramphenicol. Physiol. Plant. 88, 251-258 (1993).
Wagner, A. M.: A role for active oxygen species as second messengers in the induction of alternative oxidase gene expression in Petunia hybrida cells. FEBS Lett. 368, 339-342 (1995).
Wagner, A. M., and K. Krab: The alternative pathway in plants: role and regulation. Physiol. Plant. 95, 318-325 (1995).
Wagner, A. M., and A. L. Moore: Structure and function of the plant alternative oxidase: its putative role in the oxygen defence mechanism. Biosci. Rep. 17, 319-333 (1997).
Wagner, A. M., and M. J. Wagner: Changes in mitochondrial respiratory chain components of Petunia cells during culture in the presence of antimycin A. Plant Physiol. 115, 617-622 (1997).