Article (Scientific journals)
Enhancing The Antilisterial Effect Of Lactobacillus Curvatus Cwbi-B28 In Pork Meat And Cocultures By Limiting Bacteriocin Degradation
Kouakou, P.; Ghalfi, H.; Destain, Jacqueline et al.
2008In Meat Science, 80 (3), p. 640-648
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Keywords :
bacteriocin; Lactobacillus curvatus; Listeria monocytogenes; Commercial pork meat; Proteases
Abstract :
[en] This work focused on Listeria monocytogenes growth inhibition and growth rebound in raw and cooked pork meat inoculated with Lactobacillus curvatus strains. During storage of raw meat homogenates in the presence of the bacteriocin-producing strain Lactobacillus curvatus CWBI-B28wt, the Listeria monocytogenes cfu count was initially reduced to an undetectable level, but a growth rebound occurred after two weeks, coinciding with loss of 70% of the bacteriocin activity present at the end of week 2. The Listeria growth rebound was suppressed when proteolysis of bacteriocin was countered by the absence of proteases (bacteriocin addition to cooked meat) or the presence of 1% soy flour (added to provide competing substrates). Further experiments confirmed that bacteriocin is sensitive to the action of proteolytic enzymes isolated from both Lactobacillus curvatus CWBI-B28wt and the meat matrix. Bacteriocin proteolysis thus emerges as a cause of Listeria growth rebound.
Disciplines :
Microbiology
Animal production & animal husbandry
Author, co-author :
Kouakou, P.
Ghalfi, H.
Destain, Jacqueline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Dubois Dauphin, Robin ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Evrard, P.
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles
Language :
English
Title :
Enhancing The Antilisterial Effect Of Lactobacillus Curvatus Cwbi-B28 In Pork Meat And Cocultures By Limiting Bacteriocin Degradation
Publication date :
2008
Journal title :
Meat Science
ISSN :
0309-1740
eISSN :
1873-4138
Publisher :
Elsevier, Netherlands
Volume :
80
Issue :
3
Pages :
640-648
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
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