Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes.

Aldehyde-Lyases/isolation & purification/metabolism; Aldehydes/metabolism; Beta vulgaris/enzymology; Chromatography, High Pressure Liquid; Cytochrome P-450 Enzyme System/isolation & purification/metabolism; Hexobarbital/metabolism; Hydrogen-Ion Concentration; Linoleic Acids/metabolism; Linolenic Acids/metabolism; Lipid Peroxides/metabolism; Plant Leaves/enzymology

Abstract :

[en] Hydroperoxide lyase activity was found in sugar beet leaves. Its optimum pH and temperature were, respectively, 6.7 and 22 degrees C. Under these conditions, conversion of linolenic acid 13-hydroperoxide to cis-3-hexenal with a maximum yield of 80% was reached after only 2 min. The stability of cis-3-hexenal was improved by acidifying the reaction medium. Based on these studies, a bioprocess producing green-note aldehydes in a laboratory-scale was achieved.

Disciplines :

Chemistry
Agriculture & agronomy

Author, co-author :

Rabetafika, Holy-Nadia ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Gigot, Cédric ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Fauconnier, Marie-Laure ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Ongena, MARC ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Destain, Jacqueline ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Du Jardin, Patrick ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Wathelet, Jean-Paul ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Thonart, Philippe ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles

Language :

English

Title :

Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes.

Publication date :

2008

Journal title :

Biotechnology Letters

ISSN :

0141-5492

eISSN :

1573-6776

Publisher :

Kluwer Academic Publishers, Dordrecht, Netherlands

Volume :

Issue :

Pages :

1115-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 03 December 2009

Statistics

Number of views

225 (38 by ULiège)

Number of downloads

20 (14 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Baysal T, Dermirdöven A (2007) Lipoxygenase in fruits and vegetables: A review. Enzyme Microb Technol 40:491-496
Fauconnier M-L, Marlier M (1996) An efficient procedure for the production of fatty acid hydroperoxides from hydrolyzed flax seed oil and soybean lipoxygenase. Biotechnol Tech 10:839-844
Fauconnier M-L, Perez AG, Sanz C et al. (1997) Purification and characterization of tomato leaf (Lycopersicon esculentum Mill.) hydroperoxide lyase. J Agric Food Chem 45:4232-4236
Fukushige H, Hildebrand DF (2005) A simple and efficient system for green note compound biogenesis by use of certain lipoxygenase and hydroperoxide lyase sources. J Agric Food Chem 53:6877-6882
Hatanaka A (1993) The biogeneration of green odour by green leaves. Phytochem 34:1201-1218
Kishimoto K, Matsui K, Ozawa R et al. (2006) Components of C6-aldehyde-induced resistance in Arabidopsis thaliana against a necrotrophic fungal pathogen, Botrytis cinerea. Plant Sci 170:715-723
Marczy JSz, Németh ASz, Samu Z et al. (2002) Production of hexanal from hydrolyzed sunflower oil by lipoxygenase and hydroperoxide lyase enzymes. Biotechnol Lett 24:1673-1675
Matsui K (2006) Green leaf volatiles: hydroperoxide lyase pathway of oxylipin metabolism. Curr Opin Plant Biol 9:274-280
Németh ASz, Marczy JSz, Samu Z et al. (2004) Biocatalytic production of 2-(E)-hexenal from hydrolysed linseed oil. Enzyme Microb Technol 34:667-672
Noordermeer MA, Van der Goot W, Van Kooij AJ et al. (2002) Development of a biocatalytic process for the production of C6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase. J Agric Food Chem 50:4270-4274
Schade F, Thompson JE, Legge R (2003) Use of a plant-derived enzyme template for the production of the green-note volatile hexanal. Biotechnol and Bioeng 84:265-273
Vancanneyt G, Sanz C, Farmaki T et al. (2001) Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance. Plant Biol 98:8139-8144
Whitehead IM, Slusarenko AJ, Waspi U et al. (2001). Guava (Psidium Guajava) 13-hydroperoxide lyase and uses thereof. US Patent 6,200,794 B1, 13 mar 2001