Zanthoxylum gilletii; Secondary metabolites; Antimicrobial activity; Antibiofilm; Anti-quorum sensing; Molecular Biology; Applied Microbiology and Biotechnology; Biochemistry; General Medicine; Bioengineering; Biotechnology
Abstract :
[en] Microbial resistance is facilitated by biofilm formation and quorum-sensing mediated processes. In this work, the stem bark (ZM) and fruit extracts (ZMFT) of Zanthoxylum gilletii were subjected to column chromatography and afforded lupeol (1), 2,3-epoxy-6,7-methylenedioxyconiferyl alcohol (3), nitidine chloride (4), nitidine (7), sucrose (6) and sitosterolβ-D-glucopyranoside (2). The compounds were characterized using MS and NMR spectral data. The samples were evaluated for antimicrobial, antibiofilm and anti-quorum sensing activities. Highest antimicrobial activity was exhibited by compounds 3, 4 and 7 against Staphylococcus aureus (MIC 200 µg/mL), compounds 3 and 4 against Escherichia coli (MIC = 100 µg/mL) and compounds 4 and 7 against Candida albicans (MIC = 50 µg/mL). At MIC and sub-MIC concentrations, all samples inhibited biofilm formation by pathogens and violacein production in C. violaceum CV12472 except compound 6. Good disruption of QS-sensing in C. violaceum revealed by inhibition zone diameters were exhibited by compounds 3 (11.5 ± 0.5 mm), 4 (12.5 ± 1.5 mm), 5 (15.0 ± 0.8 mm), 7 (12.0 ± 1.5 mm) as well as the crude extracts from stem barks (16.5 ± 1.2 mm) and seeds (13.0 ± 1.4 mm). The profound inhibition of quorum sensing mediated processes in test pathogens by compounds 3, 4, 5 and 7 suggests the methylenedioxy-group that these compounds possess as the possible pharmacophore.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Nalova Ikome, Hermia
Tamfu, Alfred
Abdou, Jean
Fouotsa, Hugues
Nangmo, Pamela
Castro, Fidèle
Lah, Weyepe
Tchinda, Alembert
Ceylan, Ozgur
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Nkengfack, Augustin
Language :
English
Title :
Disruption of Biofilm Formation and Quorum Sensing in Pathogenic Bacteria by Compounds from Zanthoxylum Gilletti (De Wild) P.G. Waterman
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