[en] Cardiovascular diseases have cast a significant negative impact on the lives of millions worldwide. Over the years, extensive efforts have been dedicated to enhancing diagnostic and prognostic tools for these diseases. A growing body of evidence indicates that the angiotensin convertase enzyme (ACE) and the angiotensin convertase enzyme 2 (ACE2), and angiotensin peptide levels could hold a pivotal role in assisting clinicians with the management of cardiovascular conditions, notably hypertension and heart failure. However, despite the considerable body of knowledge in this domain, a void remains in the field of analytical methodologies for these molecules. In this study, we present a fully validated LC-MS/MS method for the precise quantitation of plasma angiotensin (1-7), (1-8), (1-9), and (1-10), following the guidelines set by the Clinical and Laboratory Standards Institute (CLSI). Our method not only enables the accurate quantification of angiotensin peptides but also provides a means to assess ACE and ACE2 activity. Remarkably, our method achieved a Lower Limit of Measurement Interval (LLMI) as low as 5 pg/mL. This has enabled the detection of angiotensin (1-7), (1-8), (1-9) and (1-10) and the accurate quantitation of angiotensin (1-7), (1-8) and (1-10) in all analyzed groups, including healthy controls, patients with high blood pressure, and patients with chronic kidney disease. To our knowledge, our method represents the most sensitive approach allowing for simultaneous quantitation of these four angiotensin peptides. A distinct advantage of our method, when compared to immunoassays, is its high sensitivity combined with comprehensive chromatographic separation of all currently known angiotensin peptides. This combination translates to an exceptional level of selectivity, underscoring the value and potential of our methodology in advancing cardiovascular disease research.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Demeuse, Justine ✱; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Huyghebaert, Loreen ✱; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Determe, William ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Schoumacher, Matthieu ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Grifnée, Elodie ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Massonnet, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Dubrowski, Thomas ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Rechchad, Marwa ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Farre Segura, Jordi ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Peeters, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Cavalier, Etienne ✱; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Le Goff, Caroline ✱; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
✱ These authors have contributed equally to this work.
Language :
English
Title :
Development and validation of an LC-MS/MS method for the simultaneous quantitation of angiotensin (1-7), (1-8), (1-9) and (1-10) in human plasma
Alternative titles :
[fr] Développement et validation d'une méthode LC-MS/MS pour la quantification simultanée de l'angiotensine (1-7), (1-8), (1-9) et (1-10) dans le plasma humain
Publication date :
25 November 2023
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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