[en] Species of Mycobacteriaceae cause disease in animals and humans, including tuberculosis and leprosy. Individuals infected with organisms in the Mycobacterium tuberculosis complex (MTBC) or non-tuberculous mycobacteria (NTM) may present identical symptoms, however the treatment for each can be different. Although the NTM infection is considered less vital due to the chronicity of the disease and the infrequency of occurrence in healthy populations, diagnosis and differentiation among Mycobacterium species currently require culture isolation, which can take several weeks. The use of volatile organic compounds (VOCs) is a promising approach for species identification and in recent years has shown promise for use in the rapid analysis of both in vitro cultures as well as ex vivo diagnosis using breath or sputum. The aim of this contribution is to analyze VOCs in the culture headspace of seven different species of mycobacteria and to define the volatilome profiles that are discriminant for each species. For the pre-concentration of VOCs, solid-phase micro-extraction (SPME) was employed and samples were subsequently analyzed using gas chromatography-quadrupole mass spectrometry (GC-qMS). A machine learning approach was applied for the selection of the 13 discriminatory features, which might represent clinically translatable bacterial biomarkers.
Disciplines :
Chemistry Microbiology
Author, co-author :
Beccaria, Marco ; Université de Liège - ULiège > Molecular Systems (MolSys) ; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Franchina, Flavio ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique ; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Nasir, Mavra; Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA
Mellors, Theodore; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Hill, Jane E; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA ; Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA
Purcaro, Giorgia ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes ; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Language :
English
Title :
Investigating Bacterial Volatilome for the Classification and Identification of Mycobacterial Species by HS-SPME-GC-MS and Machine Learning.
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