CEMIP (KIAA1199) regulates inflammation, hyperplasia and fibrosis in osteoarthritis synovial membrane

[en] Osteoarthritis (OA) synovial membrane is mainly characterized by low-grade inflammation, hyperplasia with increased cell proliferation and fibrosis. We previously underscored a critical role for CEMIP in fibrosis of OA cartilage. However, its role in OA synovial membrane remains unknown. An in vitro model with fibroblast-like synoviocytes from OA patients and an in vivo model with collagenase-induced OA mice, were used to evaluate CEMIP silencing effects on inflammation, hyperplasia and fibrosis. Our results showed that i. CEMIP expression was increased in human and mouse inflamed synovial membrane; ii. CEMIP regulated the inflammatory response pathway and inflammatory cytokines production in vitro and in vivo; iii. CEMIP induced epithelial to mesenchymal transition pathway and fibrotic markers in vitro and in vivo; iv. CEMIP increased cell proliferation and synovial hyperplasia; v. CEMIP expression was increased by inflammatory cytokines and by TGF- signaling; vi. anti-fibrotic drugs decreased CEMIP expression. All these findings highlighted the central role of CEMIP in OA synovial membrane development and underscored that targeting CEMIP could be a new therapeutic approach.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

DEROYER, Céline ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

Poulet, Christophe ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

PAULISSEN, Geneviève ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

Ciregia, Federica ; Université de Liège - ULiège > GIGA > GIGA I3 - Rheumatology

MALAISE, Olivier ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

PLENER, Zelda ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

COBRAIVILLE, Gaël ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

DANIEL, Christophe ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie de l'appareil locomoteur

GILLET, Philippe ; Université de Liège - ULiège > Département des sciences cliniques ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie de l'appareil locomoteur

MALAISE, Michel ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie ; Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie

DE SENY, Dominique ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie

Language :

English

Title :

CEMIP (KIAA1199) regulates inflammation, hyperplasia and fibrosis in osteoarthritis synovial membrane

Publication date :

2022

Journal title :

Cellular and Molecular Life Sciences

ISSN :

1420-682X

eISSN :

1420-9071

Publisher :

Birkhauser Verlag, Switzerland

Peer reviewed :

Peer Reviewed verified by ORBi

Data Set :

https://doi.org/10.1007/s00018-022-04282-6

Available on ORBi :

since 28 May 2022

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